Summary of Study ST000683
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000482. The data can be accessed directly via it's Project DOI: 10.21228/M8RG76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000683 |
| Study Title | Untargeted Lipidomics for hepatic lipid profile wild type versus knockout |
| Study Type | MS analysis |
| Study Summary | Use untargeted lipidomics to investigate differences in hepatic lipid profile between wild type and knockout mice. Mice were fed with high fat diet for 10 weeks and sacrificed under randomly fed condition. Liver were harvested freshly and frozen into liquid nitrogen immediately. Each sample was combined liver tissues from three individual mice in the same group. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 3 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000482 |
| Project DOI: | doi: 10.21228/M8RG76 |
| Project Title: | Fatty liver untargeted lipidomics |
| Project Type: | MS analysis |
| Project Summary: | Use hepatic specific knockout model to identify new lipogenic gene during hepatic steatosis |
| Institute: | University of Michigan |
| Department: | Molecular & Integrative Physiology |
| Laboratory: | Rui Lab |
| Last Name: | Rui |
| First Name: | Liangyou |
| Address: | Ann Arbor, MI |
| Email: | ruily@umich.edu |
| Phone: | 734-615-7542 |
Subject:
| Subject ID: | SU000706 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type |
|---|---|---|
| SA039031 | S00026336 | knockout |
| SA039032 | S00026335 | wild type |
| Showing results 1 to 2 of 2 |
Collection:
| Collection ID: | CO000700 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000720 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000713 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000746 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001052 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000746 |
| Num Factors: | 2 |
| Num Metabolites: | 207 |
| Rt Units: | Minutes |
| Units: | peak area |
| Analysis ID: | AN001053 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000746 |
| Num Factors: | 2 |
| Num Metabolites: | 292 |
| Rt Units: | Minutes |
| Units: | peak area |
