Summary of Study ST000684

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000483. The data can be accessed directly via it's Project DOI: 10.21228/M8MS59 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000684
Study TitleEffect of fatty acids on macrophage (wild type versus knockout) lipid levels
Study TypeMS analysis
Study SummaryMacrophages from wild type or knockout mice with various treatments [none, BSA (control), palmitate + oleate, conditioned media from adipocytes treated with either BSA or palmitate + oleate). N=4/group.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups7
Total Subjects40
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8MS59
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000483
Project DOI:doi: 10.21228/M8MS59
Project Title:Effect of fatty acids on macrophage lipid levels
Project Type:MS analysis
Project Summary:Effect of fatty acids on macrophage lipid levels
Institute:University of Michigan
Department:Internal Medicine-Nephrology
Laboratory:Pennathur Lab
Last Name:Afshinnia
First Name:Farsad
Address:Ann Arbor, MI
Email:fafshin@med.umich.edu
Phone:734-615-1293

Subject:

Subject ID:SU000707
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id genotype treatment
SA039033S00027664rage knockout BSA
SA039034S00027666rage knockout BSA
SA039035S00027665rage knockout BSA
SA039036S00027667rage knockout BSA
SA039037S00027682rage knockout conditioned media with BSA
SA039038S00027681rage knockout conditioned media with BSA
SA039039S00027680rage knockout conditioned media with BSA
SA039040S00027683rage knockout conditioned media with BSA
SA039041S00027684rage knockout conditioned media with palm + oleate
SA039042S00027686rage knockout conditioned media with palm + oleate
SA039043S00027685rage knockout conditioned media with palm + oleate
SA039044S00027687rage knockout conditioned media with palm + oleate
SA039045S00027653rage knockout none
SA039046S00027654rage knockout none
SA039047S00027655rage knockout none
SA039048S00027652rage knockout none
SA039049S00027671rage knockout palm + oleate
SA039050S00027669rage knockout palm + oleate
SA039051S00027670rage knockout palm + oleate
SA039052S00027668rage knockout palm + oleate
SA039053S00027658wild type BSA
SA039054S00027659wild type BSA
SA039055S00027657wild type BSA
SA039056S00027656wild type BSA
SA039057S00027675wild type conditioned media with BSA
SA039058S00027673wild type conditioned media with BSA
SA039059S00027674wild type conditioned media with BSA
SA039060S00027672wild type conditioned media with BSA
SA039061S00027679wild type conditioned media with palm + oleate
SA039062S00027678wild type conditioned media with palm + oleate
SA039063S00027677wild type conditioned media with palm + oleate
SA039064S00027676wild type conditioned media with palm + oleate
SA039065S00027650wild type none
SA039066S00027648wild type none
SA039067S00027649wild type none
SA039068S00027651wild type none
SA039069S00027662wild type palm + oleate
SA039070S00027661wild type palm + oleate
SA039071S00027660wild type palm + oleate
SA039072S00027663wild type palm + oleate
Showing results 1 to 40 of 40

Collection:

Collection ID:CO000701
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000721
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000714
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001054 AN001055
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000747
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000949
Analysis ID:AN001054
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000950
Analysis ID:AN001055
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  logo