Summary of Study ST000686

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000485. The data can be accessed directly via it's Project DOI: 10.21228/M8C89G This work is supported by NIH grant, U2C- DK119886.

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Study IDST000686
Study TitleLipodomics and Gestational bisphenol A (BPA) exposure
Study TypeMS analysis
Study SummaryGestational BPA exposure in sheep induces metabolic phenotype in sheep characterized by peripheral insulin resistance and increased adipocyte size. Because insulin sensitivity can be regulated by various agents including free fatty acids (FFA), we hypothesize that gestational BPA exposure alters circulating FFA inducing dyslipidemia, a marker of metabolic disorder. Because saturated FFA is associated with insulin resistance, determination of the FFA profile in these species aid in understanding the underlying mechanism. Additionally, because of the non-monotonic nature of responses to BPA exposure dose response studies are also needed. This study will therefore assess plasma lipid profile in sheep exposed to three different doses of BPA prenatally and compared with untreated control animals
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-07-05
Num Groups4
Total Subjects28
Study CommentsMetabolic disorders such as obesity and diabetes are currently widespread with epidemic proportions. Recent studies have implicated that these disorders have developmental basis due to maternal exposure to adverse insults including endocrine disruptors such as Bisphenol A (BPA). As BPA is present in maternal serum, amniotic fluid, cord blood taken at birth, placenta, colostrum and breast milk suggests that BPA has developmental impacts. In fact developmental BPA exposure have been linked to intrauterine growth restriction and low birth weight offspring, risk factors for adult cardiometabolic abnormalities. Animal models are helpful to study the effect of developmental BPA exposure and determine associated mechanisms.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8C89G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000485
Project DOI:doi: 10.21228/M8C89G
Project Title:Impact of prenatal bisphenol A (BPA) on the lipidomics of the female sheep
Project Type:MS analysis
Project Summary:Gestational exposure of bisphenol A in female sheep induces metabolic phenotype and the impact of BPA on lipidomics is being assessed to determine the mechanism that leads to altered insulin sensitivity
Institute:University of Michigan
Department:Environmental Health Sciences
Laboratory:Padmanabhan Lab
Last Name:Padmanabhan
First Name:Vasantha
Address:Ann Arbor, MI
Email:vasantha@umich.edu
Phone:734-647-0276

Subject:

Subject ID:SU000709
Subject Type:Animal
Subject Species:Ovis aries
Taxonomy ID:9940
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id BPA_DOSE
SA039090S00028709Control
SA039091S00028713Control
SA039092S00028712Control
SA039093S00028710Control
SA039094S00028711Control
SA039095S00028736High
SA039096S00028735High
SA039097S00028732High
SA039098S00028734High
SA039099S00028733High
SA039100S00028721Low
SA039101S00028719Low
SA039102S00028715Low
SA039103S00028714Low
SA039104S00028716Low
SA039105S00028717Low
SA039106S00028720Low
SA039107S00028718Low
SA039108S00028731Medium
SA039109S00028730Medium
SA039110S00028723Medium
SA039111S00028724Medium
SA039112S00028722Medium
SA039113S00028725Medium
SA039114S00028726Medium
SA039115S00028728Medium
SA039116S00028727Medium
SA039117S00028729Medium
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000703
Collection Summary:-

Treatment:

Treatment ID:TR000723
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000716
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001058 AN001059
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000749
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS000953
Analysis ID:AN001058
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS000954
Analysis ID:AN001059
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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