Summary of Study ST000689
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000488. The data can be accessed directly via it's Project DOI: 10.21228/M80031 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000689 |
| Study Title | Influence of Noxa knockdown on cell metabolism |
| Study Type | MS analysis |
| Study Summary | Stable clones of Control lentiviral (CLV) or lentiviral Noxa knockdown cells (NshLV7) (as described in Lowman et al, 2010) were counted at T-18hr and 60E6 cells were resuspended in 100ml of fresh complete medium. At T0 cells were counted again and 20E6 were collected into triplicate tubes. Cells were washed 1X with ice cold PBS and resuspended in 300ul ice cold methanol. Cells were then snap frozen in liquid nitrogen and stored at -80C. An additional 10E6 cells were collected for protein concentration and Western blot analysis. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000488 |
| Project DOI: | doi: 10.21228/M80031 |
| Project Title: | Influence of Noxa knockdown on cell metabolism |
| Project Type: | MS analysis |
| Project Summary: | Study of the influence of Noxa knockdown on cell metabolism |
| Institute: | University of Minnesota |
| Department: | Masonic Cancer Center |
| Laboratory: | Kelekar Lab |
| Last Name: | Kelekar |
| First Name: | Ameeta |
| Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
| Email: | ameeta@umn.edu |
| Phone: | 612-626-2358 |
Subject:
| Subject ID: | SU000712 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA039161 | S00020126 | - |
| SA039162 | S00013526 | Control lentivirus |
| SA039163 | S00013528 | Control lentivirus |
| SA039164 | S00013527 | Control lentivirus |
| SA039165 | S00013529 | Noxa knockdown |
| SA039166 | S00013530 | Noxa knockdown |
| SA039167 | S00013531 | Noxa knockdown |
| Showing results 1 to 7 of 7 |
Collection:
| Collection ID: | CO000706 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000726 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000719 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001063 | AN001064 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000752 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000958 |
| Analysis ID: | AN001063 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS000959 |
| Analysis ID: | AN001064 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
