Summary of Study ST000690
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000489. The data can be accessed directly via it's Project DOI: 10.21228/M8V89T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000690 |
| Study Title | Human oral keratinocytes growth and proliferation based on culture medium volume |
| Study Type | MS analysis |
| Study Summary | We seeded cells at density of 500k/T75 and cultured them at two different medium volume, one at 30ml, the other at 15ml. Culture medium is composed of 1x EDGS and 0.06mM calcium. We changed medium every day for 30ml culture, and every two days for 15ml culture. We collected spent medium when we changed medium. The collected spent medium was filtered through low protein binding 0.22um pore size filter and then stored at -80 degree C. For OA and SMT, spent medium was collected on the same day of D5 and D9 after cell seeding as marked on the tubes. For GK samples, 15ml and 30ml cultures were collected when cells reached at around 90% confluence, that is D5 for 15ml and D6 for 30 ml culture. The numbers 151, 152 were duplicated cell cultures in two T75s, same for 301 and 302. One major differences among GK, OA, and SMT is GK was from frozen cells; OA and SMT were fresh cells that were never frozen. Blank15 and Blank30 were complete medium without cells incubating in incubator for two days for Blank15 and one day for Blank30. Medium was collected in the same manner as others. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 10 |
| Total Subjects | 5 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000489 |
| Project DOI: | doi: 10.21228/M8V89T |
| Project Title: | Human oral keratinocytes growth and proliferation based on culture medium volume |
| Project Type: | MS analysis |
| Project Summary: | Using different culture medium volume to affect cultured human oral keratinocytes growth and proliferation rates |
| Institute: | University of Michigan |
| Department: | Oral & Maxillofacial Surgery |
| Laboratory: | Feinberg Lab |
| Last Name: | Feinberg |
| First Name: | Stephen |
| Address: | Ann Arbor, MI |
| Email: | sefein@umich.edu |
| Phone: | 734-763-5963 |
Subject:
| Subject ID: | SU000713 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Day | Line | Volume |
|---|---|---|---|---|
| SA039169 | S00014319 | 2 days | Blank | 15ml |
| SA039170 | S00014320 | 2 days | Blank | 30ml |
| SA039171 | S00014308 | 5 days | GK | 15ml |
| SA039172 | S00014307 | 5 days | GK | 15ml |
| SA039173 | S00014311 | 5 days | OA | 15ml |
| SA039174 | S00014313 | 5 days | OA | 30ml |
| SA039175 | S00014315 | 5 days | SMT | 15ml |
| SA039176 | S00014317 | 5 days | SMT | 30ml |
| SA039177 | S00014309 | 6 days | GK | 30ml |
| SA039178 | S00014310 | 6 days | GK | 30ml |
| SA039179 | S00014312 | 9 days | OA | 15ml |
| SA039180 | S00014314 | 9 days | OA | 30ml |
| SA039181 | S00014316 | 9 days | SMT | 15ml |
| SA039182 | S00014318 | 9 days | SMT | 30ml |
| SA039168 | S00020128 | - | - | - |
| Showing results 1 to 15 of 15 |
Collection:
| Collection ID: | CO000707 |
| Collection Summary: | - |
| Sample Type: | Keratinocytes |
Treatment:
| Treatment ID: | TR000727 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000720 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001065 | AN001066 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000753 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000960 |
| Analysis ID: | AN001065 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS000961 |
| Analysis ID: | AN001066 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
