Summary of study ST000693

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000165. The data can be accessed directly via it's Project DOI: 10.21228/M8WS31 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000693
Study TitleLanthanide-mineral induced alteration of bile acid metabolism in a murine model of steatohepatitis (part II)
Study TypeMS analysis
Study Summary120 mice (equal number of males and females) were randomized into 3 equal groups. One group is on a high-fat "Western-style" diet (HFWD) alone, a second group is on HFWD supplemented with lanthanides and calcium, and a third control group is supplemented with calcium alone. At study termination (18 months) we will harvest hepatocytes and colonic enterocytes for evaluation of epithelial gene expression patterns. We will also harvest cecal contents and feces for microbial profiling by 16S rRNA pyrosequencing. For this small pilot proposal, we wish to add an untargeted metabolomic analysis component. We will harvest liver (right medial lobe with gall bladder), serum, and feces. We will assay representative liver/gall bladder samples (8 from the HFWD group and 8 from the lanthanide/calcium supplemented group). Remaining liver samples and the serum and feces will be archived for future investigation. Liver is being targeted first since both steatohepatitis and hepatocellular carcinoma were seen in our previous study in mice on HFWD. Additionally, alterations of bile acid profiles and bile acid metabolism have been associated with both steatohepatitis and hepatic cancers.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects21
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8WS31
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000165
Project DOI:doi: 10.21228/M8WS31
Project Title:Tumor Suppression
Project Summary:Tumor suppression by diet in mice fed HFWD
Institute:University of Michigan
Department:Animal Medicine
Laboratory:Bergin Lab
Last Name:Bergin
First Name:Ingrid
Address:Ann Arbor, MI
Email:ingridbe@med.umich.edu
Phone:734-936-1701
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