Summary of Study ST000694

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000166. The data can be accessed directly via it's Project DOI: 10.21228/M8S01D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000694
Study TitleDifferences in bile acids composition between ASCL5 knockout and floxed mice.
Study TypeMS analysis
Study SummaryUnderstand the differences in Bile Acid composition between knockout and floxed mice at each intestinal segment. Also difference in metabolites between these two groups at each level of the intestine.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8S01D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000166
Project DOI:doi: 10.21228/M8S01D
Project Title:ASCL5 Bile Acids Analysis
Project Summary:Analysis of Bile Acids in Intestinal Segments of Chow Fed Male ACSL5 Knockout Mice
Institute:Tufts University
Department:Endocrinology, Diabetes, and Metabolism
Laboratory:Greenberg Lab
Last Name:Greenberg
First Name:Andrew
Address:Medford, MA
Email:andrew.greenberg@tufts.edu
Phone:617-556-3144

Subject:

Subject ID:SU000717
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Intestinal Segment
SA039266S00015224FLOX Duodenum
SA039267S00015218FLOX Duodenum
SA039268S00015209FLOX Duodenum
SA039269S00015233FLOX Duodenum
SA039270S00015215FLOX Duodenum
SA039271S00015236FLOX Duodenum
SA039272S00015239FLOX Duodenum
SA039273S00015200FLOX Duodenum
SA039274S00015211FLOX Illeum
SA039275S00015241FLOX Illeum
SA039276S00015217FLOX Illeum
SA039277S00015238FLOX Illeum
SA039278S00015235FLOX Illeum
SA039279S00015226FLOX Illeum
SA039280S00015220FLOX Illeum
SA039281S00015202FLOX Illeum
SA039282S00015219FLOX Jejunum
SA039283S00015225FLOX Jejunum
SA039284S00015237FLOX Jejunum
SA039285S00015201FLOX Jejunum
SA039286S00015216FLOX Jejunum
SA039287S00015234FLOX Jejunum
SA039288S00015210FLOX Jejunum
SA039289S00015240FLOX Jejunum
SA039265S00016589- -
SA039290S00015230Knockout Duodenum
SA039291S00015227Knockout Duodenum
SA039292S00015221Knockout Duodenum
SA039293S00015242Knockout Duodenum
SA039294S00015212Knockout Duodenum
SA039295S00015206Knockout Duodenum
SA039296S00015197Knockout Duodenum
SA039297S00015203Knockout Duodenum
SA039298S00015194Knockout Duodenum
SA039299S00015196Knockout Illeum
SA039300S00015244Knockout Illeum
SA039301S00015199Knockout Illeum
SA039302S00015229Knockout Illeum
SA039303S00015223Knockout Illeum
SA039304S00015232Knockout Illeum
SA039305S00015205Knockout Illeum
SA039306S00015214Knockout Illeum
SA039307S00015208Knockout Illeum
SA039308S00015195Knockout Jejunum
SA039309S00015213Knockout Jejunum
SA039310S00015243Knockout Jejunum
SA039311S00015222Knockout Jejunum
SA039312S00015231Knockout Jejunum
SA039313S00015228Knockout Jejunum
SA039314S00015204Knockout Jejunum
SA039315S00015207Knockout Jejunum
SA039316S00015198Knockout Jejunum
SA039317S00020138Pooled flushing Pooled flushing
Showing results 1 to 53 of 53

Collection:

Collection ID:CO000711
Collection Summary:-
Sample Type:Intestine

Treatment:

Treatment ID:TR000731
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000724
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001073 AN001074
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000757
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000968
Analysis ID:AN001073
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS000969
Analysis ID:AN001074
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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