Summary of Study ST000697

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000494. The data can be accessed directly via it's Project DOI: 10.21228/M86K78 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000697
Study TitleCytokines correlation with metabolomic profiling of psoriatic and normal skin
Study TypeMS analysis
Study SummaryThese experiments are cytokine stimulations (TNF, IL-17, IFNg) of three keratinocyte lines (HAHA, PAK and BS4). Aim is to determine the metabolic changes induced by these cytokines and correlate with metabolomic profiling of psoriatic, uninvolved and normal skin.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups9
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M86K78
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000494
Project DOI:doi: 10.21228/M86K78
Project Title:Psoriasis studies
Project Type:MS analysis
Project Summary:Metabolomics of Psoriasis
Institute:University of Michigan
Department:Dermatology
Laboratory:Gudjonsson Lab
Last Name:Gudjonsson
First Name:Johann
Address:Ann Arbor, MI
Email:johanng@med.umich.edu
Phone:734-615-4508

Subject:

Subject ID:SU000720
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell Type Stimulation
SA039435S00017245BS4 IFN-G
SA039436S00017243BS4 IL-17A
SA039437S00017242BS4 TNF-alpha
SA039438S00017244BS4 TNF-alpha+IL-17A
SA039439S00017241BS4 Untreated
SA039440S00017235HAHA IFN-G
SA039441S00017233HAHA IL-17A
SA039442S00017232HAHA TNF-alpha
SA039443S00017234HAHA TNF-alpha+IL-17A
SA039444S00017231HAHA Untreated
SA039445S00017240PAK IFN-G
SA039446S00017238PAK IL-17A
SA039447S00017237PAK TNF-alpha
SA039448S00017239PAK TNF-alpha+IL-17A
SA039449S00017236PAK Untreated
SA039450S00017526POOLED POOLED
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000714
Collection Summary:-
Sample Type:Keratinocytes

Treatment:

Treatment ID:TR000734
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000727
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001081 AN001082
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000761
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000976
Analysis ID:AN001081
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS000977
Analysis ID:AN001082
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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