Summary of Study ST000698

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000495. The data can be accessed directly via it's Project DOI: 10.21228/M82S4Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000698
Study TitleMetabolomics analysis of Sirt5 knockdown (KD) melanoma
Study TypeMS analysis
Study SummaryDetermine what kind of metabolites changing with Sirt5 KD. Con stands for the different condition of media
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects7
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M82S4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000495
Project DOI:doi: 10.21228/M82S4Z
Project Title:Analysis of Sirt5 knockdown (KD) melanoma
Project Type:MS analysis
Project Summary:Sirt5 nontageted metabolites analysis
Institute:University of Michigan
Department:UMich Pathology
Laboratory:Lombard Lab
Last Name:Beecher
First Name:Chris
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:chrisbee@med.umich.edu
Phone:734-232-0815

Subject:

Subject ID:SU000721
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Condition KD#1 KD#2
SA039452S00018257no no yes
SA039453S00018256no no yes
SA039454S00018258no no yes
SA039455S00018270no no yes
SA039456S00018269no no yes
SA039457S00018255no no yes
SA039458S00018271no no yes
SA039459S00018254no no yes
SA039460S00018272no no yes
SA039461S00018273no no yes
SA039462S00018268no yes no
SA039463S00018264no yes no
SA039464S00018265no yes no
SA039465S00018267no yes no
SA039466S00018249no yes no
SA039467S00018253no yes no
SA039468S00018252no yes no
SA039469S00018251no yes no
SA039470S00018250no yes no
SA039471S00018266no yes no
SA039451S00020150Pooled melanoma Pooled melanoma Pooled melanoma
SA039472S00018259yes no no
SA039473S00018247yes no no
SA039474S00018246yes no no
SA039475S00018245yes no no
SA039476S00018248yes no no
SA039477S00018244yes no no
SA039478S00018262yes no no
SA039479S00018261yes no no
SA039480S00018260yes no no
SA039481S00018263yes no no
Showing results 1 to 31 of 31

Collection:

Collection ID:CO000715
Collection Summary:-

Treatment:

Treatment ID:TR000735
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000728
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001083 AN001084
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000762
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000978
Analysis ID:AN001083
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS000979
Analysis ID:AN001084
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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