Summary of Study ST000699

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000496. The data can be accessed directly via it's Project DOI: 10.21228/M8Z32C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000699
Study TitleHuman vitreous amino acid & lipid analysis
Study TypeMS analysis
Study SummaryHuman vitreous was collected in the OR at time of indicated surgery. No treatments were involved. Diagnoses are: epiretinal membranes (ERM); proliferative diabetic retinopathy (PDR); retinal detachment (RD)
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8Z32C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000496
Project DOI:doi: 10.21228/M8Z32C
Project Title:Comparative Vitreous Analysis
Project Type:MS analysis
Project Summary:Human vitreous collected in OR
Institute:University of Michigan
Department:Ophthalmology
Laboratory:Gardner
Last Name:Gardner
First Name:Thomas
Address:Ann Arbor, MI
Email:tomwgard@umich.edu
Phone:734-232-8283

Subject:

Subject ID:SU000722
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diagnosis
SA039482S00018825ERM
SA039483S00018821ERM
SA039484S00018824ERM
SA039485S00018823ERM
SA039486S00018822ERM
SA039487S00018829PDR
SA039488S00018828PDR
SA039489S00018827PDR
SA039490S00018826PDR
SA039491S00018833RD
SA039492S00018834RD
SA039493S00018832RD
SA039494S00018830RD
SA039495S00018831RD
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000716
Collection Summary:-
Sample Type:Vitreous

Treatment:

Treatment ID:TR000736
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000729
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Chromatography:

Chromatography ID:CH000763
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH000764
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm
Chromatography Type:GC

Analysis:

Analysis ID:AN001085
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000763
Num Factors:3
Num Metabolites:30
Rt Units:Minutes
Units:peak area normalized
  
Analysis ID:AN001086
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000763
Num Factors:3
Num Metabolites:128
Rt Units:Minutes
Units:peak area normalized
  
Analysis ID:AN001087
Analysis Type:MS
Analysis Protocol File:A005_Amino_Acids.pdf
Instrument Name:GC_7890N MS_5975 (Agilent)
Chromatography ID:CH000764
Num Factors:3
Num Metabolites:22
Units:uM
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