Summary of study ST000701

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000148. The data can be accessed directly via it's Project DOI: 10.21228/M8V011 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000701
Study TitleDifferences in metabolism in mouse embryonic fibroblasts (control vs SCF-beta-TrCP knockout)
Study TypeMS analysis
Study SummaryMouse embryonic fibroblasts were grown in DMED supplemented with 10%FBS. The same number of cells were plated in eight 6cm dishes. When the cells were ~70% confluent, four dishes received ethanol (carrier) and the other four received tamoxifen (1uM final) to induce knockout. The cells and media were harvested 4 days later. The media harvested from control and knockout dishes will be compared with the starting medium. The cells were washed with cold PBS twice while they are attached to the dishes. The whole dish with cells attached were immediately frozen in dry ice and wrapped with aluminum foil and stored at -80C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups6
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8V011
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000148
Project DOI:doi: 10.21228/M8V011
Project Title:trcp
Project Summary:metabolic phenotypes of a clock mutant mouse
Institute:Florida State University
Department:Biomedical Sciences
Laboratory:Lee Lab
Last Name:Lee
First Name:Choogon
Address:Tallahassee, FL
Email:choogon.lee@med.fsu.edu
Phone:850-645-1478
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