Summary of Study ST000702
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000499. The data can be accessed directly via it's Project DOI: 10.21228/M8JS5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000702 |
| Study Title | NIH 3T3 fibroblast cells with Ceftriaxone Treatment |
| Study Type | MS analysis |
| Study Summary | NIH 3T3 cells were plated at 1.5million cells per 10cM dish into 12 10cm dishes. 24hrs post plating media was changed on all plates. At the time of media change, 4 dishes were treated with a final concentration of 50uM ceftriaxone (24hrs ceftriaxone n=4). 23 hrs post media change, 4 separate dishes were treated with 50uM ceftriaxone for 1hr (1hr ceftriaxone n=4). 4 dishes were untreated (0hr, basal, n=4). 24 hrs post media change, media was quickly removed from all 12 plates, each plate was rapidly washed with MilliQ H2O and rapidly frozen with liquid nitrogen poured directly into the dishes. All plates were transferred to dry ice and stored at -80 until shipped. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 3 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000499 |
| Project DOI: | doi: 10.21228/M8JS5P |
| Project Title: | NIH 3T3 fibroblast cells with Ceftriaxone Treatment |
| Project Type: | MS analysis |
| Project Summary: | 3T3 cells treated with 50uM Ceftriaxone for 24hrs, 1hr, or 0 hrs. |
| Institute: | Vanderbilt University |
| Department: | Pharmacology |
| Laboratory: | Blakely Lab |
| Last Name: | Blakely |
| First Name: | Randy |
| Address: | Nashville, TN |
| Email: | randy.blakely@vanderbilt.edu |
| Phone: | 615-936-1700 |
Subject:
| Subject ID: | SU000725 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Timepoint |
|---|---|---|
| SA039539 | S00020154 | - |
| SA039540 | S00019331 | 0hr |
| SA039541 | S00019334 | 0hr |
| SA039542 | S00019332 | 0hr |
| SA039543 | S00019333 | 0hr |
| SA039544 | S00019338 | 1hr |
| SA039545 | S00019337 | 1hr |
| SA039546 | S00019336 | 1hr |
| SA039547 | S00019335 | 1hr |
| SA039548 | S00019342 | 24hr |
| SA039549 | S00019341 | 24hr |
| SA039550 | S00019340 | 24hr |
| SA039551 | S00019339 | 24hr |
| Showing results 1 to 13 of 13 |
Collection:
| Collection ID: | CO000719 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000739 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000732 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001091 | AN001092 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000767 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000986 |
| Analysis ID: | AN001091 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS000987 |
| Analysis ID: | AN001092 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
