Summary of study ST000702

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000499. The data can be accessed directly via it's Project DOI: 10.21228/M8JS5P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000702
Study TitleNIH 3T3 fibroblast cells with Ceftriaxone Treatment
Study TypeMS analysis
Study SummaryNIH 3T3 cells were plated at 1.5million cells per 10cM dish into 12 10cm dishes. 24hrs post plating media was changed on all plates. At the time of media change, 4 dishes were treated with a final concentration of 50uM ceftriaxone (24hrs ceftriaxone n=4). 23 hrs post media change, 4 separate dishes were treated with 50uM ceftriaxone for 1hr (1hr ceftriaxone n=4). 4 dishes were untreated (0hr, basal, n=4). 24 hrs post media change, media was quickly removed from all 12 plates, each plate was rapidly washed with MilliQ H2O and rapidly frozen with liquid nitrogen poured directly into the dishes. All plates were transferred to dry ice and stored at -80 until shipped.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s).wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8JS5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000499
Project DOI:doi: 10.21228/M8JS5P
Project Title:NIH 3T3 fibroblast cells with Ceftriaxone Treatment
Project Type:MS analysis
Project Summary:3T3 cells treated with 50uM Ceftriaxone for 24hrs, 1hr, or 0 hrs.
Institute:Vanderbilt University
Department:Pharmacology
Laboratory:Blakely Lab
Last Name:Blakely
First Name:Randy
Address:Nashville, TN
Email:randy.blakely@vanderbilt.edu
Phone:615-936-1700
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