Summary of Study ST000707

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000504. The data can be accessed directly via it's Project DOI: 10.21228/M8HM3W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000707
Study TitleAmino Acid Analysis of hamster's gut contents
Study TypeMS analysis
Study SummaryHamsters were treated with Clindamycin, or a Saline, or nothing at all. After four days animals were sacrificed and their cecal contents were collected to record change in their Microbiome. Along with the Microbiome analysis we are also performing metabolomics analysis to understand the influence of change in the microbiome with the metabolic changes (if any). The cecal contents collected were frozen immediately (using liquid nitrogen) and was frozen in -80C until analysis.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HM3W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000504
Project DOI:doi: 10.21228/M8HM3W
Project Title:Amino Acid Analysis of Hamsters treated with antibiotics
Project Type:MS analysis
Project Summary:Amino Acid Analysis of Hamsters treated with antibiotics
Institute:Kansas State University
Department:Biology
Laboratory:Govind Lab
Last Name:Govind
First Name:Revathi
Address:Manhattan, KS
Email:rgovind@ksu.edu
Phone:785-532-2816

Subject:

Subject ID:SU000730
Subject Type:Animal
Subject Species:Mesocricetus auratus
Taxonomy ID:10036
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mesocricetus auratus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA039907S00020062Antibiotic treated
SA039908S00020057Antibiotic treated
SA039909S00020061Antibiotic treated
SA039910S00020063Antibiotic treated
SA039911S00020058Antibiotic treated
SA039912S00020060Antibiotic treated
SA039913S00020059Antibiotic treated
SA039914S00020072No treatment
SA039915S00020074No treatment
SA039916S00020073No treatment
SA039917S00020075No treatment
SA039918S00020071No treatment
SA039919S00020076No treatment
SA039920S00020067Saline treated
SA039921S00020065Saline treated
SA039922S00020064Saline treated
SA039923S00020066Saline treated
SA039924S00020068Saline treated
SA039925S00020069Saline treated
SA039926S00020070Saline treated
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000724
Collection Summary:-
Sample Type:Intestine

Treatment:

Treatment ID:TR000744
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000737
Sampleprep Summary:-
Sampleprep Protocol Filename:A005 - Amino Acids.pdf

Combined analysis:

Analysis ID AN001098
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000772
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-AAA Amino Acid Analysis 10m x 0.25mm
Chromatography Type:GC

MS:

MS ID:MS000993
Analysis ID:AN001098
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:A005_Amino_Acids.pdf
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