Summary of Study ST000712

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000509. The data can be accessed directly via it's Project DOI: 10.21228/M8VW91 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000712
Study TitleDiabetic heart adrenergic signaling and metabolism (STZ & Isoproterenol, High Fat)
Study TypeMS analysis
Study SummaryWe are examing the effect of both diabetes and adrenergic activation on the metabolomic profile. STZ treatment was initiated 5 months before hearts were harvested. High fat/low fat diets were given for 13 weeks. All hearts were excised in the morning. For the STZ/isoproterenol experiment, mouse anesthesia was initiated with 4% isoflurane, and maintained with 2% and a homeothermic controller. After 5 min of equilibration, control and fasted mice were injected intraperitoneally with either saline (S) or 25ug/kg isoproterenol (I). After an additional 5 min, the whole heart was quickly excised, washed with ice-cold distilled water, dried off with a chem whipe, and flash frozen in liquid nitrogen. Hearts were stored at -80, and shipped using dry ice. The hearts from the high fat diet experiement were prepared in the same way, but no injection was given and total anesthesia time was 5min instead of 10min.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups7
Total Subjects29
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VW91
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000509
Project DOI:doi: 10.21228/M8VW91
Project Title:Diabetic Heart
Project Type:MS analysis
Project Summary:Diabetic heart adrenergic signaling and metabolism
Institute:Oklahoma Medical Research Foundation
Department:Aging & Metabolism
Laboratory:Humphries Lab
Last Name:Humphries
First Name:Kenneth
Address:Oklahoma City, OK
Email:Kenneth-Humphries@omrf.org
Phone:405-271-7584

Subject:

Subject ID:SU000735
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Type
SA040080S00022386Isoproterenol Control
SA040081S00022388Isoproterenol Control
SA040082S00022387Isoproterenol Control
SA040083S00022389Isoproterenol Control
SA040084S00022392Isoproterenol STZ diabetic
SA040085S00022394Isoproterenol STZ diabetic
SA040086S00022393Isoproterenol STZ diabetic
SA040087S00022391Isoproterenol STZ diabetic
SA040088S00022390Isoproterenol STZ diabetic
SA040099S00022400none High fat diet
SA040100S00022402none High fat diet
SA040101S00022404none High fat diet
SA040102S00022401none High fat diet
SA040103S00022403none High fat diet
SA040104S00022395none Low fat diet
SA040105S00022396none Low fat diet
SA040106S00022397none Low fat diet
SA040107S00022398none Low fat diet
SA040108S00022399none Low fat diet
SA040089S00022380Saline Control
SA040090S00022378Saline Control
SA040091S00022377Saline Control
SA040092S00022376Saline Control
SA040093S00022379Saline Control
SA040094S00022381Saline STZ diabetic
SA040095S00022384Saline STZ diabetic
SA040096S00022383Saline STZ diabetic
SA040097S00022385Saline STZ diabetic
SA040098S00022382Saline STZ diabetic
Showing results 1 to 29 of 29

Collection:

Collection ID:CO000729
Collection Summary:-
Sample Type:Muscle

Treatment:

Treatment ID:TR000749
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000742
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001112
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520B QTOF
Ion Mode NEGATIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000781
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Rate:0.075 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS001007
Analysis ID:AN001112
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
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