Summary of Study ST000713

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000510. The data can be accessed directly via it's Project DOI: 10.21228/M8R68T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000713
Study TitleMetabolic profiling of cyst fluid from patients with Intraductal Pancreatic Mucinous Neoplasm
Study TypeMS analysis
Study SummaryThe pancreatic cyst fluids were collected using a syringe aspiration of the cyst fluid immediately after surgical resection of the lesion. The cyst fluid were then aliquoted and stored in -80C freezer until ready to use. We plan on performing untargeted metabolomics analysis on these pancreatic cyst fluid to find potential biomarkers that correlate with histopathological assessment and clinical behavior of these cystic lesions and thus to guide clinical management of patients.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups18
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8R68T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000510
Project DOI:doi: 10.21228/M8R68T
Project Title:Metabolic profiling of cyst fluid from patients with Intraductal Pancreatic Mucinous Neoplasm
Project Type:MS analysis
Project Summary:Identify novel cystic fluid biomarkers to guide clinical management of patients with intraductal pancreatic mucinous neoplasm (IPMN) by metabolic profiling of the cyst fluid.
Institute:University of Michigan
Department:Pathology
Laboratory:Shi Lab
Last Name:Shi
First Name:Jiaqi
Address:Ann Arbor, MI
Email:jiaqis@umich.edu
Phone:734-647-3967

Subject:

Subject ID:SU000736
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id level of dysplasia malignant transformation
SA040111S00022448HGD No
SA040110S00022447HGD & PDA yes
SA040109S00022449HGD & PDA Yes
SA040112S00022438LGD No
SA040113S00022444LGD No
SA040114S00022441LGD No
SA040115S00022439LGD No
SA040116S00022443LGD No
SA040117S00022442LGD No
SA040118S00022445N/A No
SA040119S00022440N/A No
SA040120S00022446N/A No
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000730
Collection Summary:-
Sample Type:Cyst fluid

Treatment:

Treatment ID:TR000750
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000743
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001113 AN001114
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000782
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000783
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001008
Analysis ID:AN001113
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:RP Neg Untargeted_2016_Agilent_5_2mm
Processing Parameters File:-
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001009
Analysis ID:AN001114
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Acquisition Parameters File:RP Pos Untargeted_2016_Agilent_6_2mm
Processing Parameters File:-
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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