Summary of Study ST000714

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000511. The data can be accessed directly via it's Project DOI: 10.21228/M8MD5H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000714
Study TitleEffect of genetic lesions on glioblastoma (NP, NPA, NPAI, NS)
Study TypeMS analysis
Study SummaryThe cells in this experiment are primary cells derived from tumors with various genetic lesions. The purpose of this experiment is to determine the effect of the genetic lesion IDH1m (mutant isocitrate dehydrogenase) on the metabolic state of the cell. Four replicates of NPA versus four replicates of NPAI suspension cell neurospheres will be compared in an untargeted manner to identify all possible metabolic differences between the two types of genetic lesions.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8MD5H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000511
Project DOI:doi: 10.21228/M8MD5H
Project Title:Young adult glioblastoma
Project Type:MS analysis
Project Summary:Effect of genetic lesions on glioblastoma
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000737
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id IDh1 mutation
SA040121S00022549NPA
SA040122S00022552NPA
SA040123S00022551NPA
SA040124S00022550NPA
SA040125S00022556NPAI
SA040126S00022553NPAI
SA040127S00022554NPAI
SA040128S00022555NPAI
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000731
Collection Summary:-
Sample Type:Tumor cells

Treatment:

Treatment ID:TR000751
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000744
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001117 AN001118
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000784
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001012
Analysis ID:AN001117
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001013
Analysis ID:AN001118
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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