Summary of Study ST000720

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000517. The data can be accessed directly via it's Project DOI: 10.21228/M8TW9Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000720
Study TitleMetabolomics of lean males given water, antibiotic cocktail (ANMV), or neomycin then exposed to ozone
Study TypeMS analysis
Study SummaryMice were given either water, an antibiotic cocktail (ANMV), or Neomycin in their drinking water for two weeks to reduce bacterial load, and then exposed to air or to ozone (2ppm for 3hrs). 24 hours later, pulmonary mechanics and airway responsiveness was assessed, and blood was collected via cardiac puncture. Serum was isolated and immediately stored at -80 C for further analysis.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups7
Total Subjects21
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8TW9Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000517
Project DOI:doi: 10.21228/M8TW9Q
Project Title:Metabolomics of lean males given water, antibiotic cocktail (ANMV), or neomycin then exposed to ozone
Project Type:MS analysis
Project Summary:Lean males given water, ANMV, or Neomycin then exposed to ozone
Institute:Harvard School of Public Health
Department:Environmental Health
Laboratory:Shore Lab
Last Name:Shore
First Name:Stephanie
Address:Boston, MA
Email:sshore@hsph.harvard.edu
Phone:617-432-0199

Subject:

Subject ID:SU000743
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA040373S00025625ANMV
SA040374S00025624ANMV
SA040375S00025627ANMV
SA040376S00025628ANMV
SA040377S00025629ANMV
SA040378S00025623ANMV
SA040379S00025626ANMV
SA040380S00025633Neomycin
SA040381S00025632Neomycin
SA040382S00025634Neomycin
SA040383S00025635Neomycin
SA040384S00025636Neomycin
SA040385S00025631Neomycin
SA040386S00025630Neomycin
SA040387S00025621Water
SA040388S00025617Water
SA040389S00025616Water
SA040390S00025620Water
SA040391S00025618Water
SA040392S00025619Water
SA040393S00025622Water
Showing results 1 to 21 of 21

Collection:

Collection ID:CO000737
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000757
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000750
Sampleprep Summary:-
Sampleprep Protocol Filename:A007_-_Short_Chain_Fatty_Acids.pdf

Combined analysis:

Analysis ID AN001126
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000792
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS001021
Analysis ID:AN001126
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:A007_-_Short_Chain_Fatty_Acids.pdf
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