Summary of Study ST000723

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000520. The data can be accessed directly via it's Project DOI: 10.21228/M8FQ1J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000723
Study TitleAnalysis of SCFA in the fecal matter of wt and Orai1kO mice
Study TypeMS analysis
Study SummaryThe gut microbiome participates in numerous physiological functions and is believed to be shaped by the host intestine. We report here an unsuspected prominent role of Orai1-mediated pancreatic acinar cell exocytosis in shaping the microbiome. Deletion of Orai1 in pancreatic acini of mature mice maintained on solid diet resulted in 60-70% mortality. The mice have severe bacterial burden with dysbiosis resulting in systemic inflammation and death. This occurs despite prominent Paneth cell hyperplasia and activation of the intestinal innate immune response. Secretion of and bacterial killing by pancreatic defensins are markedly reduced, resulting in microbiota with decreased diversity and increased pathogenic bacteria. Survival and weight gain are not rescued by digestive enzyme supplements, but are by broad-spectrum antibiotics and purified liquid diet. These findings reveal an unexpected profound role of pancreatic secretion in shaping the microbiome
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8FQ1J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000520
Project DOI:doi: 10.21228/M8FQ1J
Project Title:Analysis of SCFA in the fecal matter of wt and Orai1kO mice
Project Type:MS analysis
Project Summary:Orai1 mediated pancreatic secretion is critical for gut innate immunity
Institute:National Institutes of Health
Department:NIDCR/MPTB
Laboratory:Muallem Lab
Last Name:Muallem
First Name:Shmuel
Address:Bethesda, Maryland
Phone:301-471-3027

Subject:

Subject ID:SU000746
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Genotype
SA040666S00026178liquid diet Orai1Ko
SA040667S00026180liquid diet Orai1Ko
SA040668S00026179liquid diet Orai1Ko
SA040669S00026181liquid diet Orai1Ko
SA040670S00026182liquid diet Orai1Ko
SA040671S00026174liquid diet wt
SA040672S00026175liquid diet wt
SA040673S00026177liquid diet wt
SA040674S00026173liquid diet wt
SA040675S00026176liquid diet wt
SA040676S00026172solid diet Orai1Ko
SA040677S00026168solid diet Orai1Ko
SA040678S00026169solid diet Orai1Ko
SA040679S00026167solid diet Orai1Ko
SA040680S00026170solid diet Orai1Ko
SA040681S00026171solid diet Orai1Ko
SA040682S00026163solid diet wt
SA040683S00026162solid diet wt
SA040684S00026164solid diet wt
SA040685S00026166solid diet wt
SA040686S00026161solid diet wt
SA040687S00026165solid diet wt
Showing results 1 to 22 of 22

Collection:

Collection ID:CO000740
Collection Summary:-
Sample Type:Feces

Treatment:

Treatment ID:TR000760
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000753
Sampleprep Summary:-
Sampleprep Protocol Filename:A007_-_Short_Chain_Fatty_Acids.pdf

Combined analysis:

Analysis ID AN001133
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000796
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
Chromatography Type:GC

MS:

MS ID:MS001028
Analysis ID:AN001133
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:A007_-_Short_Chain_Fatty_Acids.pdf
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