Summary of Study ST000732
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000529. The data can be accessed directly via it's Project DOI: 10.21228/M89101 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000732 |
| Study Title | Acylcarnitine profile in fast-refed livers |
| Study Type | MS analysis |
| Study Summary | The aim of this study is to determine whether changes in CoA degradation affect the composition of the acylcarntine pool in the liver. 7 wild-type and 7 Nudt7 knockout male mice (10-16 weeks of age) on a mixed background were fasted for 24h and allowed to refeed for 12-14hours before removing the liver. The liver samples were flash-frozen and stored at -80C until used. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 4 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000529 |
| Project DOI: | doi: 10.21228/M89101 |
| Project Title: | Determining the role of Nudt7 in liver metabolism |
| Project Type: | MS analysis |
| Project Summary: | Determining the role of Nudt7 in liver metabolism |
| Institute: | West Virginia University |
| Department: | Biochemistry and Molecular Biology |
| Laboratory: | Leonardi Lab |
| Last Name: | Leonardi |
| First Name: | Roberta |
| Address: | Morgantown, WV |
| Email: | roleonardi@hsc.wvu.edu |
| Phone: | 304-293-7591 |
Subject:
| Subject ID: | SU000755 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Hours after start of re-feeding | Nudt7 Genotype |
|---|---|---|---|
| SA040936 | S00028683 | 12h | KO |
| SA040937 | S00028684 | 12h | KO |
| SA040938 | S00028682 | 12h | WT |
| SA040939 | S00028681 | 12h | WT |
| SA040940 | S00028692 | 14h | KO |
| SA040941 | S00028690 | 14h | KO |
| SA040942 | S00028694 | 14h | KO |
| SA040943 | S00028691 | 14h | KO |
| SA040944 | S00028693 | 14h | KO |
| SA040945 | S00028688 | 14h | WT |
| SA040946 | S00028685 | 14h | WT |
| SA040947 | S00028686 | 14h | WT |
| SA040948 | S00028687 | 14h | WT |
| SA040949 | S00028689 | 14h | WT |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000749 |
| Collection Summary: | - |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR000769 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000762 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A008_-_Acyl_Carnitines.pdf |
Chromatography:
| Chromatography ID: | CH000806 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Rate: | 0.250 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001146 |
| Analysis Type: | MS |
| Analysis Protocol File: | A008_-_Acyl_Carnitines.pdf |
| Instrument Name: | 6490A QQQ LC/MS (Agilent) |
| Chromatography ID: | CH000806 |
| Num Factors: | 4 |
| Num Metabolites: | 27 |
| Units: | nmol/mg |
