Summary of Study ST000732

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000529. The data can be accessed directly via it's Project DOI: 10.21228/M89101 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000732
Study TitleAcylcarnitine profile in fast-refed livers
Study TypeMS analysis
Study SummaryThe aim of this study is to determine whether changes in CoA degradation affect the composition of the acylcarntine pool in the liver. 7 wild-type and 7 Nudt7 knockout male mice (10-16 weeks of age) on a mixed background were fasted for 24h and allowed to refeed for 12-14hours before removing the liver. The liver samples were flash-frozen and stored at -80C until used.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M89101
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000529
Project DOI:doi: 10.21228/M89101
Project Title:Determining the role of Nudt7 in liver metabolism
Project Type:MS analysis
Project Summary:Determining the role of Nudt7 in liver metabolism
Institute:West Virginia University
Department:Biochemistry and Molecular Biology
Laboratory:Leonardi Lab
Last Name:Leonardi
First Name:Roberta
Address:Morgantown, WV
Email:roleonardi@hsc.wvu.edu
Phone:304-293-7591

Subject:

Subject ID:SU000755
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Hours after start of re-feeding Nudt7 Genotype
SA040936S0002868312h KO
SA040937S0002868412h KO
SA040938S0002868212h WT
SA040939S0002868112h WT
SA040940S0002869214h KO
SA040941S0002869014h KO
SA040942S0002869414h KO
SA040943S0002869114h KO
SA040944S0002869314h KO
SA040945S0002868814h WT
SA040946S0002868514h WT
SA040947S0002868614h WT
SA040948S0002868714h WT
SA040949S0002868914h WT
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000749
Collection Summary:-
Sample Type:Liver

Treatment:

Treatment ID:TR000769
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000762
Sampleprep Summary:-
Sampleprep Protocol Filename:A008_-_Acyl_Carnitines.pdf

Combined analysis:

Analysis ID AN001146
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode POSITIVE
Units nmol/mg

Chromatography:

Chromatography ID:CH000806
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001041
Analysis ID:AN001146
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
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