Summary of Study ST000734

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000531. The data can be accessed directly via it's Project DOI: 10.21228/M81H4W This work is supported by NIH grant, U2C- DK119886.


Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000734
Study TitleMeasuring SCFA in mice gut following colonization by C. difficile
Study TypeMS analysis
Study Summary3 cages of 5-8 week old C57B/6 mice were placed on the antibiotic cefoperazone for 10 days followed by 2 days of reovery on plain water. One cage remained on plain water the entire time. Of the three antibiotic treated cages. One was mock infected (uninfected control), one was infected with C. difficile strain VPI 10463, while the other was infected with C. difficile strain 630. Twenty-four hours after the infection the mice were eutanized and the ceal content was collected and snap frozen in liquid nitrogen. Samples were stored in the -80 until analysis.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups4
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000531
Project DOI:doi: 10.21228/M81H4W
Project Title:SCFA During Infection
Project Type:MS analysis
Project Summary:Measuring SCFA following 24hr colonization by either C. difficile strain 630 or strain VPI. Compared to uninfected mice.
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Young Lab
Last Name:Young
First Name:Vincent
Address:Ann Arbor, MI


Subject ID:SU000757
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal


Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id CEFOPERAZONE Infection
SA040994S00022595NO NO
SA040995S00022597NO NO
SA040996S00022596NO NO
SA040997S00022608YES C.difficile 630
SA040998S00022609YES C.difficile 630
SA040999S00022611YES C.difficile 630
SA041000S00022612YES C.difficile 630
SA041001S00022610YES C.difficile 630
SA041002S00022607YES C.difficile VPI 10463
SA041003S00022606YES C.difficile VPI 10463
SA041004S00022604YES C.difficile VPI 10463
SA041005S00022605YES C.difficile VPI 10463
SA041006S00022603YES C.difficile VPI 10463
SA041007S00022599YES NO
SA041008S00022598YES NO
SA041009S00022601YES NO
SA041010S00022602YES NO
SA041011S00022600YES NO
Showing results 1 to 18 of 18


Collection ID:CO000751
Collection Summary:-
Sample Type:Intestine


Treatment ID:TR000771
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000764
Sampleprep Summary:-
Sampleprep Protocol Filename:A007_-_Short_Chain_Fatty_Acids.pdf

Combined analysis:

Analysis ID AN001148
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Units nmol/mg


Chromatography ID:CH000808
Instrument Name:Agilent 7890N
Column Name:Phenomenex Zebron ZB-WAXplus (30m x 0.25mm,0.25um)
Chromatography Type:GC


MS ID:MS001043
Analysis ID:AN001148
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Analysis Protocol File:A007_-_Short_Chain_Fatty_Acids.pdf