Summary of Study ST000735

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000532. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ1K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000735
Study TitleMurine bile acid profiles during CDI R20291 infection
Study TypeMS analysis
Study SummaryWe are interested in understanding the bile acid profiles in cecal content from mice challeneged with R20291 C. difficle spores. We are also interested in the bile acid profiles of mice that are administered Ursodiol (UDCA) or vehcile (corn oil) during C. difficile infection. In vitro Ursodiol (UDCA) has significant impacts on germination and growth of C. difficile R20291.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups19
Total Subjects89
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8WQ1K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000532
Project DOI:doi: 10.21228/M8WQ1K
Project Title:Murine Bile Acid Profiles during R20291 Infection
Project Type:MS analysis
Project Summary:Targeted bile acid metabolomics
Institute:North Carolina State University
Department:Population Health and Pathobiology
Laboratory:Theriot Lab
Last Name:Theriot
First Name:Casey
Address:Raleigh, NC
Email:caseythe@umich.edu
Phone:734-763-4133

Subject:

Subject ID:SU000758
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id SEX TIMEPOINT TREATMENT
SA041014S00024745F Day 0 no treatment
SA041015S00024747F Day 0 no treatment
SA041016S00024746F Day 0 no treatment
SA041017S00024748F Day 0 no treatment
SA041012S00024754F Day 0 UDCA
SA041013S00024752F Day 0 UDCA
SA041018S00024751F Day 0 vehicle
SA041019S00024749F Day 0 vehicle
SA041022S00024758F Day 2 no treatment
SA041023S00024756F Day 2 no treatment
SA041020S00024764F Day 2 UDCA
SA041021S00024766F Day 2 UDCA
SA041024S00024762F Day 2 vehicle
SA041025S00024760F Day 2 vehicle
SA041028S00024769F Day 4 no treatment
SA041029S00024771F Day 4 no treatment
SA041026S00024777F Day 4 UDCA
SA041027S00024779F Day 4 UDCA
SA041030S00024775F Day 4 vehicle
SA041031S00024773F Day 4 vehicle
SA041034S00024783F Day 7 no treatment
SA041035S00024781F Day 7 no treatment
SA041032S00024788F Day 7 UDCA
SA041033S00024790F Day 7 UDCA
SA041036S00024786F Day 7 vehicle
SA041037S00024785F Day 7 vehicle
SA041038S00024831F NA post antibiotics
SA041039S00024830F NA post antibiotics
SA041040S00024827F NA pre antibiotics
SA041041S00024826F NA pre antibiotics
SA041042S00024753M Day 0 UDCA
SA041043S00024755M Day 0 UDCA
SA041044S00024750M Day 0 vehicle
SA041047S00024757M Day 2 no treatment
SA041048S00024759M Day 2 no treatment
SA041045S00024767M Day 2 UDCA
SA041046S00024765M Day 2 UDCA
SA041049S00024768M Day 2 vehicle
SA041050S00024763M Day 2 vehicle
SA041051S00024761M Day 2 vehicle
SA041054S00024770M Day 4 no treatment
SA041055S00024772M Day 4 no treatment
SA041052S00024778M Day 4 UDCA
SA041053S00024780M Day 4 UDCA
SA041056S00024774M Day 4 vehicle
SA041057S00024776M Day 4 vehicle
SA041060S00024784M Day 7 no treatment
SA041061S00024782M Day 7 no treatment
SA041058S00024789M Day 7 UDCA
SA041059S00024791M Day 7 UDCA
SA041062S00024787M Day 7 vehicle
SA041063S00024832M NA post antibiotics
SA041064S00024833M NA post antibiotics
SA041065S00024828M NA pre antibiotics
SA041066S00024829M NA pre antibiotics
SA041067S00024795NA Day 0 day0 no challenge
SA041068S00024793NA Day 0 day0 no challenge
SA041069S00024794NA Day 0 day0 no challenge
SA041070S00024792NA Day 0 day0 no challenge
SA041071S00024824NA Day 14 day14 post challenge
SA041072S00024823NA Day 14 day14 post challenge
SA041073S00024821NA Day 14 day14 post challenge
SA041074S00024822NA Day 14 day14 post challenge
SA041075S00024820NA Day 14 day14 post challenge
SA041076S00024825NA Day 14 day14 post challenge
SA041077S00024804NA Day 2 day2 post challenge
SA041078S00024802NA Day 2 day2 post challenge
SA041079S00024803NA Day 2 day2 post challenge
SA041080S00024805NA Day 2 day2 post challenge
SA041081S00024806NA Day 2 day2 post challenge
SA041082S00024807NA Day 2 day2 post challenge
SA041083S00024813NA Day 4 day4 post challenge
SA041084S00024811NA Day 4 day4 post challenge
SA041085S00024809NA Day 4 day4 post challenge
SA041086S00024808NA Day 4 day4 post challenge
SA041087S00024812NA Day 4 day4 post challenge
SA041088S00024810NA Day 4 day4 post challenge
SA041089S00024799NA Day 7 day7 no challenge
SA041090S00024800NA Day 7 day7 no challenge
SA041091S00024798NA Day 7 day7 no challenge
SA041092S00024797NA Day 7 day7 no challenge
SA041093S00024796NA Day 7 day7 no challenge
SA041094S00024801NA Day 7 day7 no challenge
SA041095S00024819NA Day 7 day7 post challenge
SA041096S00024815NA Day 7 day7 post challenge
SA041097S00024816NA Day 7 day7 post challenge
SA041098S00024818NA Day 7 day7 post challenge
SA041099S00024817NA Day 7 day7 post challenge
SA041100S00024814NA Day 7 day7 post challenge
Showing results 1 to 89 of 89

Collection:

Collection ID:CO000752
Collection Summary:-
Sample Type:Intestine

Treatment:

Treatment ID:TR000772
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000765
Sampleprep Summary:-
Sampleprep Protocol Filename:A014 - Bile Acids.pdf

Combined analysis:

Analysis ID AN001149
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Acquity BEH C18 (50 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode NEGATIVE
Units ug/100 mg

Chromatography:

Chromatography ID:CH000809
Instrument Name:Agilent
Column Name:Waters Acquity BEH C18 (50 x 2mm,1.7um)
Flow Rate:200ul/min
Solvent A:75% acetonitrile/40% methanol; 10mM ammonium acetate
Solvent B:100% water; 10 mM ammonium acetate, pH 8.0
Chromatography Type:Reversed phase

MS:

MS ID:MS001044
Analysis ID:AN001149
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A014_BileAcids.pdf
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