Summary of Study ST000735
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000532. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ1K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000735 |
| Study Title | Murine bile acid profiles during CDI R20291 infection |
| Study Type | MS analysis |
| Study Summary | We are interested in understanding the bile acid profiles in cecal content from mice challeneged with R20291 C. difficle spores. We are also interested in the bile acid profiles of mice that are administered Ursodiol (UDCA) or vehcile (corn oil) during C. difficile infection. In vitro Ursodiol (UDCA) has significant impacts on germination and growth of C. difficile R20291. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 19 |
| Total Subjects | 89 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000532 |
| Project DOI: | doi: 10.21228/M8WQ1K |
| Project Title: | Murine Bile Acid Profiles during R20291 Infection |
| Project Type: | MS analysis |
| Project Summary: | Targeted bile acid metabolomics |
| Institute: | North Carolina State University |
| Department: | Population Health and Pathobiology |
| Laboratory: | Theriot Lab |
| Last Name: | Theriot |
| First Name: | Casey |
| Address: | Raleigh, NC |
| Email: | caseythe@umich.edu |
| Phone: | 734-763-4133 |
Subject:
| Subject ID: | SU000758 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | SEX | TIMEPOINT | TREATMENT |
|---|---|---|---|---|
| SA041014 | S00024745 | F | Day 0 | no treatment |
| SA041015 | S00024747 | F | Day 0 | no treatment |
| SA041016 | S00024746 | F | Day 0 | no treatment |
| SA041017 | S00024748 | F | Day 0 | no treatment |
| SA041012 | S00024754 | F | Day 0 | UDCA |
| SA041013 | S00024752 | F | Day 0 | UDCA |
| SA041018 | S00024751 | F | Day 0 | vehicle |
| SA041019 | S00024749 | F | Day 0 | vehicle |
| SA041022 | S00024758 | F | Day 2 | no treatment |
| SA041023 | S00024756 | F | Day 2 | no treatment |
| SA041020 | S00024764 | F | Day 2 | UDCA |
| SA041021 | S00024766 | F | Day 2 | UDCA |
| SA041024 | S00024762 | F | Day 2 | vehicle |
| SA041025 | S00024760 | F | Day 2 | vehicle |
| SA041028 | S00024769 | F | Day 4 | no treatment |
| SA041029 | S00024771 | F | Day 4 | no treatment |
| SA041026 | S00024777 | F | Day 4 | UDCA |
| SA041027 | S00024779 | F | Day 4 | UDCA |
| SA041030 | S00024775 | F | Day 4 | vehicle |
| SA041031 | S00024773 | F | Day 4 | vehicle |
| SA041034 | S00024783 | F | Day 7 | no treatment |
| SA041035 | S00024781 | F | Day 7 | no treatment |
| SA041032 | S00024788 | F | Day 7 | UDCA |
| SA041033 | S00024790 | F | Day 7 | UDCA |
| SA041036 | S00024786 | F | Day 7 | vehicle |
| SA041037 | S00024785 | F | Day 7 | vehicle |
| SA041038 | S00024831 | F | NA | post antibiotics |
| SA041039 | S00024830 | F | NA | post antibiotics |
| SA041040 | S00024827 | F | NA | pre antibiotics |
| SA041041 | S00024826 | F | NA | pre antibiotics |
| SA041042 | S00024753 | M | Day 0 | UDCA |
| SA041043 | S00024755 | M | Day 0 | UDCA |
| SA041044 | S00024750 | M | Day 0 | vehicle |
| SA041047 | S00024757 | M | Day 2 | no treatment |
| SA041048 | S00024759 | M | Day 2 | no treatment |
| SA041045 | S00024767 | M | Day 2 | UDCA |
| SA041046 | S00024765 | M | Day 2 | UDCA |
| SA041049 | S00024768 | M | Day 2 | vehicle |
| SA041050 | S00024763 | M | Day 2 | vehicle |
| SA041051 | S00024761 | M | Day 2 | vehicle |
| SA041054 | S00024770 | M | Day 4 | no treatment |
| SA041055 | S00024772 | M | Day 4 | no treatment |
| SA041052 | S00024778 | M | Day 4 | UDCA |
| SA041053 | S00024780 | M | Day 4 | UDCA |
| SA041056 | S00024774 | M | Day 4 | vehicle |
| SA041057 | S00024776 | M | Day 4 | vehicle |
| SA041060 | S00024784 | M | Day 7 | no treatment |
| SA041061 | S00024782 | M | Day 7 | no treatment |
| SA041058 | S00024789 | M | Day 7 | UDCA |
| SA041059 | S00024791 | M | Day 7 | UDCA |
| SA041062 | S00024787 | M | Day 7 | vehicle |
| SA041063 | S00024832 | M | NA | post antibiotics |
| SA041064 | S00024833 | M | NA | post antibiotics |
| SA041065 | S00024828 | M | NA | pre antibiotics |
| SA041066 | S00024829 | M | NA | pre antibiotics |
| SA041067 | S00024795 | NA | Day 0 | day0 no challenge |
| SA041068 | S00024793 | NA | Day 0 | day0 no challenge |
| SA041069 | S00024794 | NA | Day 0 | day0 no challenge |
| SA041070 | S00024792 | NA | Day 0 | day0 no challenge |
| SA041071 | S00024824 | NA | Day 14 | day14 post challenge |
| SA041072 | S00024823 | NA | Day 14 | day14 post challenge |
| SA041073 | S00024821 | NA | Day 14 | day14 post challenge |
| SA041074 | S00024822 | NA | Day 14 | day14 post challenge |
| SA041075 | S00024820 | NA | Day 14 | day14 post challenge |
| SA041076 | S00024825 | NA | Day 14 | day14 post challenge |
| SA041077 | S00024804 | NA | Day 2 | day2 post challenge |
| SA041078 | S00024802 | NA | Day 2 | day2 post challenge |
| SA041079 | S00024803 | NA | Day 2 | day2 post challenge |
| SA041080 | S00024805 | NA | Day 2 | day2 post challenge |
| SA041081 | S00024806 | NA | Day 2 | day2 post challenge |
| SA041082 | S00024807 | NA | Day 2 | day2 post challenge |
| SA041083 | S00024813 | NA | Day 4 | day4 post challenge |
| SA041084 | S00024811 | NA | Day 4 | day4 post challenge |
| SA041085 | S00024809 | NA | Day 4 | day4 post challenge |
| SA041086 | S00024808 | NA | Day 4 | day4 post challenge |
| SA041087 | S00024812 | NA | Day 4 | day4 post challenge |
| SA041088 | S00024810 | NA | Day 4 | day4 post challenge |
| SA041089 | S00024799 | NA | Day 7 | day7 no challenge |
| SA041090 | S00024800 | NA | Day 7 | day7 no challenge |
| SA041091 | S00024798 | NA | Day 7 | day7 no challenge |
| SA041092 | S00024797 | NA | Day 7 | day7 no challenge |
| SA041093 | S00024796 | NA | Day 7 | day7 no challenge |
| SA041094 | S00024801 | NA | Day 7 | day7 no challenge |
| SA041095 | S00024819 | NA | Day 7 | day7 post challenge |
| SA041096 | S00024815 | NA | Day 7 | day7 post challenge |
| SA041097 | S00024816 | NA | Day 7 | day7 post challenge |
| SA041098 | S00024818 | NA | Day 7 | day7 post challenge |
| SA041099 | S00024817 | NA | Day 7 | day7 post challenge |
| SA041100 | S00024814 | NA | Day 7 | day7 post challenge |
| Showing results 1 to 89 of 89 |
Collection:
| Collection ID: | CO000752 |
| Collection Summary: | - |
| Sample Type: | Intestine |
Treatment:
| Treatment ID: | TR000772 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000765 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A014 - Bile Acids.pdf |
Combined analysis:
| Analysis ID | AN001149 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent |
| Column | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490A QQQ |
| Ion Mode | NEGATIVE |
| Units | ug/100 mg |
Chromatography:
| Chromatography ID: | CH000809 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
| Flow Rate: | 200ul/min |
| Solvent A: | 75% acetonitrile/40% methanol; 10mM ammonium acetate |
| Solvent B: | 100% water; 10 mM ammonium acetate, pH 8.0 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001044 |
| Analysis ID: | AN001149 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A014_BileAcids.pdf |
