Summary of Study ST000740

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000534. The data can be accessed directly via it's Project DOI: 10.21228/M8N67H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000740
Study TitleQuantitating the baseline fatty acid composition in phospholipids in subgroups of tumor cells
Study TypeMS analysis
Study SummaryDetermine the capacity of control and experimental cells to convert stearate to oleate. Determine free 13C stearate/oleate or that in PC, PE and PI.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups8
Total Subjects10
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8N67H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000534
Project DOI:doi: 10.21228/M8N67H
Project Title:Quantitating the baseline FA composition in phospholipids in subgroups of tumor cells
Project Type:MS analysis
Project Summary:Quantitating the baseline FA composition in phospholipids in subgroups of tumor cells
Institute:Cincinnati Children's Hospital Medical Center
Department:Oncology
Laboratory:Dasgupta Lab
Last Name:Dasgupta
First Name:Biplab
Address:Cincinnati, OH
Email:biplab.dasgupta@cchmc.org
Phone:513-803-1370

Subject:

Subject ID:SU000763
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Type
SA041158S000263330.2% O2 WT
SA041159S000263340.2% O2 WT
SA041160S000263320.5% O2 WT
SA041161S000263310.5% O2 WT
SA041162S000263301% O2 WT
SA041163S000263291% O2 WT
SA041164S00026328none HD
SA041165S00026325none HSCD
SA041166S00026326none LSCD
SA041167S00026327none WT
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000757
Collection Summary:-
Sample Type:Tumor cells

Treatment:

Treatment ID:TR000777
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000770
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001154
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890N
Column Agilent HP5-MS (15m × 0.25mm, 0.25 um)
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5975
Ion Mode POSITIVE
Units pmol/mg protein

Chromatography:

Chromatography ID:CH000814
Instrument Name:Agilent 7890N
Column Name:Agilent HP5-MS (15m × 0.25mm, 0.25 um)
Chromatography Type:GC

MS:

MS ID:MS001049
Analysis ID:AN001154
Instrument Name:Agilent 5975
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Analysis Protocol File:A011_-_Glycolysis-TCA-nucleotides.pdf
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