Summary of Study ST000741
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000535. The data can be accessed directly via it's Project DOI: 10.21228/M8HH47 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000741 |
| Study Title | Metabolite-phenotype link in X-linked Adrenoleukodystrophy (fibroblast cell culture) |
| Study Type | MS analysis |
| Study Summary | Untargeted metabolomics of control, AMN and ALD patient-derived fibroblasts. Fibroblasts cultured in DMEM with 15%FBS and antibiotic (pen/strep) in 100mm petridishes (1million/dish). After 24h cultures supplemented with fresh media (DMEM+15%FBS+antibiotic) for 3hr |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 4 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000535 |
| Project DOI: | doi: 10.21228/M8HH47 |
| Project Title: | Metabolite-phenotype link in X-linked Adrenoleukodystrophy |
| Project Type: | MS analysis |
| Project Summary: | Untargeted metabolomics of control, AMN and ALD patient-derived fibroblasts |
| Institute: | Henry Ford Health System |
| Department: | Neurology |
| Laboratory: | Singh Lab |
| Last Name: | Singh |
| First Name: | Jaspreet |
| Address: | Detroit, MI |
| Email: | jsingh2@hfhs.org |
| Phone: | 313-916-7725 |
Subject:
| Subject ID: | SU000764 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | subject type |
|---|---|---|
| SA041168 | S00016575 | Healthy control |
| SA041169 | S00016570 | Healthy control |
| SA041170 | S00016574 | Healthy control |
| SA041171 | S00016573 | Healthy control |
| SA041172 | S00016571 | Healthy control |
| SA041173 | S00016572 | Healthy control |
| SA041174 | S00016580 | Mild disease |
| SA041175 | S00016581 | Mild disease |
| SA041176 | S00016579 | Mild disease |
| SA041177 | S00016578 | Mild disease |
| SA041178 | S00016576 | Mild disease |
| SA041179 | S00016577 | Mild disease |
| SA041180 | S00017166 | POOLED |
| SA041181 | S00016587 | Severe disease |
| SA041182 | S00016586 | Severe disease |
| SA041183 | S00016584 | Severe disease |
| SA041184 | S00016582 | Severe disease |
| SA041185 | S00016583 | Severe disease |
| SA041186 | S00016585 | Severe disease |
| Showing results 1 to 19 of 19 |
Collection:
| Collection ID: | CO000758 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000778 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000771 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001155 | AN001156 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000815 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001050 |
| Analysis ID: | AN001155 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001051 |
| Analysis ID: | AN001156 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
