Summary of Study ST000743
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000536. The data can be accessed directly via it's Project DOI: 10.21228/M8CQ3P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000743 |
| Study Title | Metabolomics of bariatric weight loss |
| Study Type | MS analysis |
| Study Summary | Human patients were enrolled in a bariatric weight loss clinic and followed. Patients were male and female, normoglycemic,pre-diabetic and diabetic and with and without neuropathy. One 500 ul aliquot will be submitted for both untargeted metabolomics and shotgun lipidomics. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 7 |
| Total Subjects | 3 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000536 |
| Project DOI: | doi: 10.21228/M8CQ3P |
| Project Title: | Callaghan Bariatric plasma baseline |
| Project Type: | MS analysis |
| Project Summary: | Obesity study with intervention |
| Institute: | University of Michigan |
| Department: | Neurology |
| Laboratory: | Feldman Lab |
| Last Name: | Feldman |
| First Name: | Eva |
| Address: | Ann Arbor, MI |
| Email: | efeldman@umich.edu |
| Phone: | 734-763-7274 |
Subject:
| Subject ID: | SU000766 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Gender | Glycemic status | Neuropathy |
|---|---|---|---|---|
| SA041227 | S00025579 | female | diabetic | Neuropathy |
| SA041228 | S00025577 | female | diabetic | Neuropathy |
| SA041229 | S00025578 | female | diabetic | Neuropathy |
| SA041230 | S00025568 | female | diabetic | Neuropathy |
| SA041231 | S00025580 | female | diabetic | no neuropathy |
| SA041232 | S00025573 | female | diabetic | no neuropathy |
| SA041233 | S00025571 | female | diabetic | no neuropathy |
| SA041234 | S00025574 | female | diabetic | no neuropathy |
| SA041235 | S00025570 | female | normoglycemic | Neuropathy |
| SA041236 | S00025581 | female | normoglycemic | no neuropathy |
| SA041237 | S00025575 | male | diabetic | Neuropathy |
| SA041238 | S00025576 | male | diabetic | no neuropathy |
| SA041239 | S00025572 | male | pre-diabetic | Neuropathy |
| SA041240 | S00025569 | male | pre-diabetic | no neuropathy |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000760 |
| Collection Summary: | - |
Treatment:
| Treatment ID: | TR000780 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000773 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001159 | AN001160 | AN001161 | AN001162 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Triple TOF | Triple TOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000817 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000818 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001054 |
| Analysis ID: | AN001159 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001055 |
| Analysis ID: | AN001160 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001056 |
| Analysis ID: | AN001161 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001057 |
| Analysis ID: | AN001162 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
