Summary of Study ST000745
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000538. The data can be accessed directly via it's Project DOI: 10.21228/M84687 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000745 |
| Study Title | Metabolomics of mice inoculated with human microbiota |
| Study Type | MS analysis |
| Study Summary | Mice were inoculated with human microbiota. Peripheral blood was collected using superficial temporal phlebotomy into a tube containing EDTA-K2. Tubes were then inverted 5 times and placed on ice for no longer than one hour. Samples were centrifuged at 1500rcf for 15 minutes. Supernatant was transferred and quantified with a pipette. Tubes were placed on dry ice for between 10 minutes to an hour andl stored at -80C until shipped on dry ice. All steps during processing were performed in batches containing mulitple treatment groups. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 31 |
| Total Subjects | 26 |
| Study Comments | All samples contain EDTA-K2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000538 |
| Project DOI: | doi: 10.21228/M84687 |
| Project Title: | Metabolomics of mice inoculated with human microbiota |
| Project Type: | MS analysis |
| Project Summary: | Metabolomics of mice inoculated with human microbiota |
| Institute: | Cornell University |
| Department: | Microbiology |
| Laboratory: | Ley Lab |
| Last Name: | Ley |
| First Name: | Ruth |
| Address: | Ithaca, NY |
| Email: | rel222@cornell.edu |
| Phone: | 607-255-4954 |
Subject:
| Subject ID: | SU000768 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | AMY1 Group | Day |
|---|---|---|---|
| SA041268 | S00014395 | high | Day 35 |
| SA041269 | S00014390 | high | Day 35 |
| SA041270 | S00014378 | high | Day 35 |
| SA041271 | S00014402 | high | Day 35 |
| SA041272 | S00014399 | high | Day 35 |
| SA041273 | S00014400 | high | Day 35 |
| SA041274 | S00014388 | high | Day 35 |
| SA041275 | S00014398 | high | Day 35 |
| SA041276 | S00014389 | high | Day 35 |
| SA041277 | S00014383 | high | Day 35 |
| SA041278 | S00014379 | high | Day 35 |
| SA041279 | S00014420 | high | Day 6 |
| SA041280 | S00014404 | high | Day 6 |
| SA041281 | S00014422 | high | Day 6 |
| SA041282 | S00014418 | high | Day 6 |
| SA041283 | S00014406 | high | Day 6 |
| SA041284 | S00014416 | high | Day 6 |
| SA041285 | S00014412 | high | Day 6 |
| SA041286 | S00014410 | high | Day 6 |
| SA041287 | S00014408 | high | Day 6 |
| SA041288 | S00014414 | high | Day 6 |
| SA041289 | S00014401 | low | Day 35 |
| SA041290 | S00014385 | low | Day 35 |
| SA041291 | S00014384 | low | Day 35 |
| SA041292 | S00014391 | low | Day 35 |
| SA041293 | S00014397 | low | Day 35 |
| SA041294 | S00014387 | low | Day 35 |
| SA041295 | S00014386 | low | Day 35 |
| SA041296 | S00014393 | low | Day 35 |
| SA041297 | S00014394 | low | Day 35 |
| SA041298 | S00014381 | low | Day 35 |
| SA041299 | S00014380 | low | Day 35 |
| SA041300 | S00014417 | low | Day 6 |
| SA041301 | S00014423 | low | Day 6 |
| SA041302 | S00014419 | low | Day 6 |
| SA041303 | S00014421 | low | Day 6 |
| SA041304 | S00014413 | low | Day 6 |
| SA041305 | S00014405 | low | Day 6 |
| SA041306 | S00014403 | low | Day 6 |
| SA041307 | S00014415 | low | Day 6 |
| SA041308 | S00014407 | low | Day 6 |
| SA041309 | S00014409 | low | Day 6 |
| SA041310 | S00014411 | low | Day 6 |
| SA041311 | S00014396 | medium | Day 35 |
| SA041312 | S00014392 | medium | Day 35 |
| SA041313 | S00014382 | medium | Day 35 |
| SA041267 | S00020130 | Pooled | Pooled |
| Showing results 1 to 47 of 47 |
Collection:
| Collection ID: | CO000762 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000782 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000775 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Chromatography:
| Chromatography ID: | CH000821 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001167 |
| Analysis Type: | MS |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| Instrument Name: | 6530 Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000821 |
| Num Factors: | 6 |
| Num Metabolites: | 61 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Units: | peak area |
| Analysis ID: | AN001168 |
| Analysis Type: | MS |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| Instrument Name: | 6530 Q-TOF LC/MS (Agilent) |
| Chromatography ID: | CH000821 |
| Num Factors: | 6 |
| Num Metabolites: | 42 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Units: | peak area |
