Summary of Study ST000745

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000538. The data can be accessed directly via it's Project DOI: 10.21228/M84687 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000745
Study TitleMetabolomics of mice inoculated with human microbiota
Study TypeMS analysis
Study SummaryMice were inoculated with human microbiota. Peripheral blood was collected using superficial temporal phlebotomy into a tube containing EDTA-K2. Tubes were then inverted 5 times and placed on ice for no longer than one hour. Samples were centrifuged at 1500rcf for 15 minutes. Supernatant was transferred and quantified with a pipette. Tubes were placed on dry ice for between 10 minutes to an hour andl stored at -80C until shipped on dry ice. All steps during processing were performed in batches containing mulitple treatment groups.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups31
Total Subjects26
Study CommentsAll samples contain EDTA-K2
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M84687
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000538
Project DOI:doi: 10.21228/M84687
Project Title:Metabolomics of mice inoculated with human microbiota
Project Type:MS analysis
Project Summary:Metabolomics of mice inoculated with human microbiota
Institute:Cornell University
Department:Microbiology
Laboratory:Ley Lab
Last Name:Ley
First Name:Ruth
Address:Ithaca, NY
Email:rel222@cornell.edu
Phone:607-255-4954

Subject:

Subject ID:SU000768
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id AMY1 Group Day
SA041268S00014395high Day 35
SA041269S00014390high Day 35
SA041270S00014378high Day 35
SA041271S00014402high Day 35
SA041272S00014399high Day 35
SA041273S00014400high Day 35
SA041274S00014388high Day 35
SA041275S00014398high Day 35
SA041276S00014389high Day 35
SA041277S00014383high Day 35
SA041278S00014379high Day 35
SA041279S00014420high Day 6
SA041280S00014404high Day 6
SA041281S00014422high Day 6
SA041282S00014418high Day 6
SA041283S00014406high Day 6
SA041284S00014416high Day 6
SA041285S00014412high Day 6
SA041286S00014410high Day 6
SA041287S00014408high Day 6
SA041288S00014414high Day 6
SA041289S00014401low Day 35
SA041290S00014385low Day 35
SA041291S00014384low Day 35
SA041292S00014391low Day 35
SA041293S00014397low Day 35
SA041294S00014387low Day 35
SA041295S00014386low Day 35
SA041296S00014393low Day 35
SA041297S00014394low Day 35
SA041298S00014381low Day 35
SA041299S00014380low Day 35
SA041300S00014417low Day 6
SA041301S00014423low Day 6
SA041302S00014419low Day 6
SA041303S00014421low Day 6
SA041304S00014413low Day 6
SA041305S00014405low Day 6
SA041306S00014403low Day 6
SA041307S00014415low Day 6
SA041308S00014407low Day 6
SA041309S00014409low Day 6
SA041310S00014411low Day 6
SA041311S00014396medium Day 35
SA041312S00014392medium Day 35
SA041313S00014382medium Day 35
SA041267S00020130Pooled Pooled
Showing results 1 to 47 of 47

Collection:

Collection ID:CO000762
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000782
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000775
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001167 AN001168
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000821
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001062
Analysis ID:AN001167
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001063
Analysis ID:AN001168
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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