Summary of Study ST000748

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000539. The data can be accessed directly via it's Project DOI: 10.21228/M80H36 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000748
Study TitlePlacental cells (BeWo) phthalate exposure metabolomics
Study TypeMS analysis
Study SummaryBeWo cells were plated in 100mm x 20mm cell culture plates and allowed to attach and acclimate for 24 hours. Cell culture media was then replaced with media containing mono(2-ethylhexyl)phthalate (MEHP) at either 90 or 180 micromolar concentrations or vehicle control (DMSO, 0.05% by volume). Cells were exposed for 24 hours followed by collection of cell media which was flash frozen in liquid nitrogen and then placed on dry ice. Cells were washed with sodium acetate and then immediately flash frozen by addition of ~8mL of liquid nitrogen to tissue culture plate. All samples were stored at -80 degrees C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups5
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M80H36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000539
Project DOI:doi: 10.21228/M80H36
Project Title:Placental cells (BeWo cell line) exposure to phthalate esters
Project Type:MS analysis
Project Summary:Placental cells (BeWo cell line) were exposed to phthalate esters or vehicle control, metabolomics changes will investigated 24 hours post exposure
Institute:University of Michigan
Department:Environmental Health
Laboratory:Loch-Caruso Lab
Last Name:Loch-Caruso
First Name:Rita
Address:Ann Arbor, MI
Email:rlc@umich.edu
Phone:734-936-1256

Subject:

Subject ID:SU000771
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id MEHP concentration Sample type
SA041428S00022590180 Cells
SA041429S00022591180 Cells
SA041430S00022592180 Cells
SA041431S00022583180 Culture Media
SA041432S00022582180 Culture Media
SA041433S00022581180 Culture Media
SA041434S0002258990 Cells
SA041435S0002258790 Cells
SA041436S0002258890 Cells
SA041437S0002258090 Culture Media
SA041438S0002257990 Culture Media
SA041439S0002257890 Culture Media
SA041422S00022586- Cells
SA041423S00022585- Cells
SA041424S00022584- Cells
SA041425S00022576- Culture Media
SA041426S00022575- Culture Media
SA041427S00022577- Culture Media
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000765
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000785
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000778
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001172 AN001173
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode NEGATIVE POSITIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000824
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001067
Analysis ID:AN001172
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001068
Analysis ID:AN001173
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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