Summary of study ST000748

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000539. The data can be accessed directly via it's Project DOI: 10.21228/M80H36 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000748
Study TitlePlacental cells (BeWo) phthalate exposure metabolomics
Study TypeMS analysis
Study SummaryBeWo cells were plated in 100mm x 20mm cell culture plates and allowed to attach and acclimate for 24 hours. Cell culture media was then replaced with media containing mono(2-ethylhexyl)phthalate (MEHP) at either 90 or 180 micromolar concentrations or vehicle control (DMSO, 0.05% by volume). Cells were exposed for 24 hours followed by collection of cell media which was flash frozen in liquid nitrogen and then placed on dry ice. Cells were washed with sodium acetate and then immediately flash frozen by addition of ~8mL of liquid nitrogen to tissue culture plate. All samples were stored at -80 degrees C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups5
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M80H36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000539
Project DOI:doi: 10.21228/M80H36
Project Title:Placental cells (BeWo cell line) exposure to phthalate esters
Project Type:MS analysis
Project Summary:Placental cells (BeWo cell line) were exposed to phthalate esters or vehicle control, metabolomics changes will investigated 24 hours post exposure
Institute:University of Michigan
Department:Environmental Health
Laboratory:Loch-Caruso Lab
Last Name:Loch-Caruso
First Name:Rita
Address:Ann Arbor, MI
Email:rlc@umich.edu
Phone:734-936-1256
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