Summary of Study ST000749

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000540. The data can be accessed directly via it's Project DOI: 10.21228/M8VT19 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000749
Study TitleRat amniotic fluid metabolomics
Study TypeMS analysis
Study SummaryPregnant rats were treated for 5 days with 100mg/kg/day DEHP or vehicle control (corn oil) via a wafer which the rats consumed orally. After the fifth treatment, rats were euthanized and uterine horns were dissected. Amniotic fluid was collected from the gestational sac via syringe and collected into 1.5mL tubes. Tubes were then stored at -80 celsius
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects2
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8VT19
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000540
Project DOI:doi: 10.21228/M8VT19
Project Title:Amniotic fluid metabolomics
Project Type:MS analysis
Project Summary:Pregnant rats were exposed to phthalate (DEHP) or control via diet and amniotic fluid was collected from amnions of treated and untreated rats
Institute:University of Michigan
Department:Environmental Health
Laboratory:Loch-Caruso Lab
Last Name:Loch-Caruso
First Name:Rita
Address:Ann Arbor, MI
Email:rlc@umich.edu
Phone:734-936-1256

Subject:

Subject ID:SU000772
Subject Type:Rat
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammals

Factors:

Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA041440S00026010Control
SA041441S00026011Control
SA041442S00026012DEHP
SA041443S00026013DEHP
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000766
Collection Summary:-
Sample Type:Amniotic fluid

Treatment:

Treatment ID:TR000786
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000779
Sampleprep Summary:-
Sampleprep Protocol Filename:A003_-_Untargeted_Metabolomics.pdf

Combined analysis:

Analysis ID AN001174 AN001175
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH000825
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001069
Analysis ID:AN001174
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
  
MS ID:MS001070
Analysis ID:AN001175
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A003_-_Untargeted_Metabolomics.pdf
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