Summary of Study ST000750
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000540. The data can be accessed directly via it's Project DOI: 10.21228/M8VT19 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000750 |
| Study Title | Rat amniotic fluid metabolomics (part II) |
| Study Type | MS analysis |
| Study Summary | Pregnant rats were treated for 5 days with 100mg/kg/day DEHP or vehicle control (corn oil) via a wafer which the rats consumed orally. After the fifth treatment, rats were euthanized and uterine horns were dissected. Amniotic fluid was collected from the gestational sac via syringe and collected into 1.5mL tubes. Tubes were then stored at -80 celsius. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000540 |
| Project DOI: | doi: 10.21228/M8VT19 |
| Project Title: | Amniotic fluid metabolomics |
| Project Type: | MS analysis |
| Project Summary: | Pregnant rats were exposed to phthalate (DEHP) or control via diet and amniotic fluid was collected from amnions of treated and untreated rats |
| Institute: | University of Michigan |
| Department: | Environmental Health |
| Laboratory: | Loch-Caruso Lab |
| Last Name: | Loch-Caruso |
| First Name: | Rita |
| Address: | Ann Arbor, MI |
| Email: | rlc@umich.edu |
| Phone: | 734-936-1256 |
Subject:
| Subject ID: | SU000773 |
| Subject Type: | Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA041444 | S00027730 | Control |
| SA041445 | S00027734 | Control |
| SA041446 | S00027733 | Control |
| SA041447 | S00027731 | Control |
| SA041448 | S00027732 | Control |
| SA041449 | S00027739 | DEHP treated |
| SA041450 | S00027738 | DEHP treated |
| SA041451 | S00027735 | DEHP treated |
| SA041452 | S00027736 | DEHP treated |
| SA041453 | S00027737 | DEHP treated |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000767 |
| Collection Summary: | - |
| Sample Type: | Amniotic fluid |
Treatment:
| Treatment ID: | TR000787 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000780 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001176 | AN001177 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000826 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001071 |
| Analysis ID: | AN001176 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001072 |
| Analysis ID: | AN001177 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
