Summary of Study ST000756
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000545. The data can be accessed directly via it's Project DOI: 10.21228/M87083 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000756 |
| Study Title | Metabolomics of cilia and modulation of renal microcirculation |
| Study Type | MS analysis |
| Study Summary | Explore and explain the mechanism of regulation of metabolism biomarkers to modulate renal microcirculation. 1 - let rats rest for 5 days. 2 - test basal glucose and plasma. Store plasma in -20C for one day then transfer to -80. 3 - inject STZ 50mg/kg ip. 4 - after 3 days, check for increased glucose and extract plasma for early stage diabetic sample >300 mg/dl. Store in -20C and then -80C. 5 - one month after initial injection, extract plasma and store in -20C and then -80C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 6 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000545 |
| Project DOI: | doi: 10.21228/M87083 |
| Project Title: | Cilia and Modulation of Renal Microcirculation |
| Project Type: | MS analysis |
| Project Summary: | Explore and explain the mechanism of regulation of metabolism biomarkers to modulate renal microcirculation |
| Institute: | University of South Florida |
| Department: | Molecular Pharmacology & Physiology |
| Laboratory: | Liu Lab |
| Last Name: | Liu |
| First Name: | Ruisheng |
| Address: | Tampa, FL |
| Email: | ruisheng@health.usf.edu |
| Phone: | 813-396-9107 |
Subject:
| Subject ID: | SU000779 |
| Subject Type: | Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Days post-STZ |
|---|---|---|
| SA041592 | S00016983 | - |
| SA041593 | S00016982 | - |
| SA041594 | S00016981 | - |
| SA041595 | S00016986 | 3 |
| SA041596 | S00016985 | 3 |
| SA041597 | S00016984 | 3 |
| SA041598 | S00016989 | 30 |
| SA041599 | S00016988 | 30 |
| SA041600 | S00016987 | 30 |
| SA041601 | S00017328 | POOLED |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000773 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000793 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000786 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001186 | AN001187 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000833 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001081 |
| Analysis ID: | AN001186 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001082 |
| Analysis ID: | AN001187 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
