Summary of Study ST000757
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000546. The data can be accessed directly via it's Project DOI: 10.21228/M8397K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000757 |
| Study Title | Tubuloglomerular feedback and salt-sensitive hypertension |
| Study Type | MS analysis |
| Study Summary | Collect whole blood in tubes containing EDTA, gently invert tubes 10 times and cool as soon as practical on ice. Centrifuge to separate plasma as soon as practical, transfer to appropriately labeled tubes, and freeze immediately. I test basal glucose and plasma store plasma in -20C for one day then transfer to -80C. The next day, inject STZ 60 mg/kg ip. After 3 days, check for increased glucose and extract plasma for early stage diabetic sample >300 mg/dl. Store in -20C and then -80C. 30 days after initial injection, extract plasma and store in -20C and then -80C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 9 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000546 |
| Project DOI: | doi: 10.21228/M8397K |
| Project Title: | Tubuloglomerular feedback and salt-sensitive hypertension |
| Project Type: | MS analysis |
| Project Summary: | When TGF changes, does metabolics change |
| Institute: | University of South Florida |
| Department: | Molecular Pharmacology & Physiology |
| Laboratory: | Liu Lab |
| Last Name: | Liu |
| First Name: | Ruisheng |
| Address: | Tampa, FL |
| Email: | ruisheng@health.usf.edu |
| Phone: | 813-396-9107 |
Subject:
| Subject ID: | SU000780 |
| Subject Type: | Rat |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
Subject type: Rat; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Days | Gene | STZ injection |
|---|---|---|---|---|
| SA041605 | S00019351 | 30 days | non wild type | yes |
| SA041606 | S00019348 | 30 days | non wild type | yes |
| SA041607 | S00019345 | 30 days | non wild type | yes |
| SA041602 | S00019344 | 3 days | non wild type | yes |
| SA041603 | S00019350 | 3 days | non wild type | yes |
| SA041604 | S00019346 | 3 days | wild type | no |
| SA041608 | S00019347 | NA | non wild type | yes |
| SA041609 | S00019349 | NA | wild type | no |
| SA041610 | S00019343 | NA | wild type | no |
| SA041611 | S00020155 | Pooled | Pooled plasma | Pooled plasma |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000774 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000794 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000787 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001188 | AN001189 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000834 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001083 |
| Analysis ID: | AN001188 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001084 |
| Analysis ID: | AN001189 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
