Summary of Study ST000763
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000551. The data can be accessed directly via it's Project DOI: 10.21228/M8FH50 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000763 |
Study Title | Untargeted metabolomics and lipidomics of scleroderma PAH discovery cohort |
Study Type | MS analysis |
Study Summary | This experiment was performed using the following cohorts: 1) healthy controls, 2) patients with scleroderma at low risk for pulmonary hypertension, 3) pateints with scleroderma at high risk for pulmonary hypertension who underwent a catheterization and were found to have normal pressures, borderline elevated pressures or pulmonary arterial hypertensino (PAH). Whole blood was drawn directly into EDTA tubes at rest and again at peak exercise for each human subject. The samples were processed to plasma by MCRU and stored at -80 in the Biorepository. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2017-06-19 |
Num Groups | 11 |
Total Subjects | 5 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-12-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000551 |
Project DOI: | doi: 10.21228/M8FH50 |
Project Title: | Untargeted and lipidomics_scleroderma_PAH |
Project Type: | MS analysis |
Project Summary: | Untargeted/lipidomics analysis |
Institute: | University of Michigan |
Department: | Internal Medicine-Cardiology |
Laboratory: | Visovatti Lab |
Last Name: | Visovatti |
First Name: | Scott |
Address: | Ann Arbor, MI |
Email: | shv@med.umich.edu |
Phone: | 734-657-6917 |
Subject:
Subject ID: | SU000786 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
mb_sample_id | local_sample_id | Exercise | Group | Sex | Type |
---|---|---|---|---|---|
SA041771 | S00021447 | No | Borderline Pressures | Female | Cath |
SA041772 | S00021449 | No | Borderline Pressures | Female | Cath |
SA041773 | S00021453 | No | Borderline Pressures | Female | Cath |
SA041774 | S00021358 | No | Borderline Pressures | Female | Cath |
SA041775 | S00021271 | No | Borderline Pressures | Female | Cath |
SA041776 | S00021368 | No | Borderline Pressures | Female | Cath |
SA041777 | S00021364 | No | Borderline Pressures | Female | Cath |
SA041778 | S00021285 | No | Borderline Pressures | Female | Cath |
SA041779 | S00021279 | No | Borderline Pressures | Female | Cath |
SA041780 | S00021257 | No | Borderline Pressures | Female | Cath |
SA041781 | S00021269 | No | Borderline Pressures | Female | Cath |
SA041782 | S00021349 | No | Borderline Pressures | Female | Cath |
SA041783 | S00021378 | No | Borderline Pressures | Female | Cath |
SA041784 | S00021345 | No | Borderline Pressures | Female | Cath |
SA041785 | S00021265 | No | Borderline Pressures | Female | Cath |
SA041786 | S00021267 | No | Borderline Pressures | Female | Cath |
SA041787 | S00021291 | No | Borderline Pressures | Female | Cath |
SA041788 | S00021411 | No | Borderline Pressures | Female | Cath |
SA041789 | S00021301 | No | Borderline Pressures | Male | Cath |
SA041790 | S00021374 | No | Borderline Pressures | Male | Cath |
SA041791 | S00021305 | No | Borderline Pressures | Male | Cath |
SA041792 | S00021389 | No | Healthy | Female | Non-invasive |
SA041793 | S00021311 | No | Healthy | Female | Non-invasive |
SA041794 | S00021387 | No | Healthy | Female | Non-invasive |
SA041795 | S00021309 | No | Healthy | Female | Non-invasive |
SA041796 | S00021356 | No | Healthy | Female | Non-invasive |
SA041797 | S00021339 | No | Healthy | Female | Non-invasive |
SA041798 | S00021327 | No | Healthy | Female | Non-invasive |
SA041799 | S00021329 | No | Healthy | Female | Non-invasive |
SA041800 | S00021337 | No | Healthy | Female | Non-invasive |
SA041801 | S00021331 | No | Healthy | Female | Non-invasive |
SA041802 | S00021325 | No | Healthy | Female | Non-invasive |
SA041803 | S00021323 | No | Healthy | Female | Non-invasive |
SA041804 | S00021362 | No | Healthy | Female | Non-invasive |
SA041805 | S00021315 | No | Healthy | Female | Non-invasive |
SA041806 | S00021317 | No | Healthy | Female | Non-invasive |
SA041807 | S00021391 | No | Healthy | Female | Non-invasive |
SA041808 | S00021321 | No | Healthy | Female | Non-invasive |
SA041809 | S00021313 | No | Healthy | Female | Non-invasive |
SA041810 | S00021401 | No | Healthy | Female | Non-invasive |
SA041811 | S00021427 | No | Healthy | Female | Non-invasive |
SA041812 | S00021425 | No | Healthy | Female | Non-invasive |
SA041813 | S00021423 | No | Healthy | Female | Non-invasive |
SA041814 | S00021429 | No | Healthy | Female | Non-invasive |
SA041815 | S00021437 | No | Healthy | Female | Non-invasive |
SA041816 | S00021469 | No | Healthy | Female | Non-invasive |
SA041817 | S00021273 | No | Healthy | Female | Non-invasive |
SA041818 | S00021275 | No | Healthy | Female | Non-invasive |
SA041819 | S00021415 | No | Healthy | Female | Non-invasive |
SA041820 | S00021435 | No | Healthy | Female | Non-invasive |
SA041821 | S00021403 | No | Healthy | Female | Non-invasive |
SA041822 | S00021399 | No | Healthy | Female | Non-invasive |
SA041823 | S00021397 | No | Healthy | Female | Non-invasive |
SA041824 | S00021333 | No | Healthy | Female | Non-invasive |
SA041825 | S00021319 | No | Healthy | Male | Non-invasive |
SA041826 | S00021354 | No | Healthy | Male | Non-invasive |
SA041827 | S00021335 | No | Healthy | Male | Non-invasive |
SA041828 | S00021277 | No | Healthy | Male | Non-invasive |
SA041829 | S00021457 | No | LowRisk | Female | Non-invasive |
SA041830 | S00021461 | No | LowRisk | Female | Non-invasive |
SA041831 | S00021395 | No | LowRisk | Female | Non-invasive |
SA041832 | S00021471 | No | LowRisk | Female | Non-invasive |
SA041833 | S00021467 | No | LowRisk | Female | Non-invasive |
SA041834 | S00021465 | No | LowRisk | Female | Non-invasive |
SA041835 | S00021455 | No | LowRisk | Female | Non-invasive |
SA041836 | S00021459 | No | LowRisk | Female | Non-invasive |
SA041837 | S00021445 | No | LowRisk | Female | Non-invasive |
SA041838 | S00021382 | No | LowRisk | Female | Non-invasive |
SA041839 | S00021413 | No | LowRisk | Female | Non-invasive |
SA041840 | S00021376 | No | LowRisk | Female | Non-invasive |
SA041841 | S00021431 | No | LowRisk | Female | Non-invasive |
SA041842 | S00021433 | No | LowRisk | Female | Non-invasive |
SA041843 | S00021441 | No | LowRisk | Female | Non-invasive |
SA041844 | S00021393 | No | LowRisk | Female | Non-invasive |
SA041845 | S00021372 | No | LowRisk | Female | Non-invasive |
SA041846 | S00021366 | No | LowRisk | Female | Non-invasive |
SA041847 | S00021293 | No | LowRisk | Female | Non-invasive |
SA041848 | S00021287 | No | LowRisk | Female | Non-invasive |
SA041849 | S00021473 | No | LowRisk | Male | Non-invasive |
SA041850 | S00021405 | No | LowRisk | Male | Non-invasive |
SA041851 | S00021303 | No | Normal Pressures | Female | Cath |
SA041852 | S00021299 | No | Normal Pressures | Female | Cath |
SA041853 | S00021351 | No | Normal Pressures | Female | Cath |
SA041854 | S00021409 | No | Normal Pressures | Female | Cath |
SA041855 | S00021380 | No | Normal Pressures | Female | Cath |
SA041856 | S00021370 | No | Normal Pressures | Female | Cath |
SA041857 | S00021261 | No | Normal Pressures | Female | Cath |
SA041858 | S00021421 | No | Normal Pressures | Female | Cath |
SA041859 | S00021263 | No | Normal Pressures | Female | Cath |
SA041860 | S00021289 | No | Normal Pressures | Male | Cath |
SA041861 | S00021307 | No | PAH | Female | Cath |
SA041862 | S00021295 | No | PAH | Female | Cath |
SA041863 | S00021283 | No | PAH | Female | Cath |
SA041864 | S00021439 | No | PAH | Female | Cath |
SA041865 | S00021407 | No | PAH | Female | Cath |
SA041866 | S00021297 | No | PAH | Female | Cath |
SA041867 | S00021419 | No | PAH | Female | Cath |
SA041868 | S00021386 | No | PAH | Female | Cath |
SA041869 | S00021443 | No | PAH | Female | Cath |
SA041870 | S00021384 | No | PAH | Female | Cath |
Collection:
Collection ID: | CO000780 |
Collection Summary: | - |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000800 |
Treatment Summary: | - |
Sample Preparation:
Sampleprep ID: | SP000793 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
Analysis ID | AN001201 | AN001202 | AN001203 | AN001204 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent | Agilent | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | Triple TOF | Triple TOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
Units | peak area normalized | peak area normalized | peak area normalized | peak area normalized |
Chromatography:
Chromatography ID: | CH000842 |
Instrument Name: | Agilent |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000843 |
Instrument Name: | Shimadzu CTO-20A Nexera X2 |
Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
Column Temperature: | 55 |
Flow Rate: | 0.400uL/min |
Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001096 |
Analysis ID: | AN001201 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
MS ID: | MS001097 |
Analysis ID: | AN001202 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
MS ID: | MS001098 |
Analysis ID: | AN001203 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
MS ID: | MS001099 |
Analysis ID: | AN001204 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |