Summary of Study ST000767
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000553. The data can be accessed directly via it's Project DOI: 10.21228/M86095 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000767 |
| Study Title | Metabolite analysis of regional neural stem/progenitor cells |
| Study Type | MS analysis |
| Study Summary | The developing mammalian brain generates a variety of Neural Progenitor Cells (NPCs). Primary NPCs throughout the neuraxis are derived from the ventricular zone. Intermediate progenitor cells (IPCs) are produced uniquely in the telencephalon and contribute extensively to the neurons that comprise the cerebral cortex and basal ganglia. It is known that the fate of the diverse NPC populations is determined by the interplay of transcription factors and regulation by regional humoral cues. However, despite our recent appreciation that nutrient-regulated intracellular metabolic milieu (pO2, energy, and redox state) significantly influence cell fate, an unexplored area is whether NPCs have intrinsic metabolic identity, and if so, the mechanism by which molecular metabolism contributes to brain development.Little is known however, if intrinsic differences in cellular metabolism of regional NPCs make certain NPCs susceptible while others resistant to genetic and environmental insults. We conjectured that regional (fore-and hindbrain) NPCs are metabolically distinct. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000553 |
| Project DOI: | doi: 10.21228/M86095 |
| Project Title: | Metabolic uniqueness of neural stem/progenitor cells Studies |
| Project Type: | MS analysis |
| Project Summary: | Cell-intrinsic metabolic uniqueness of regional neural stem/progenitor cells |
| Institute: | Cincinnati Children's Hospital Medical Center |
| Department: | Oncology |
| Laboratory: | Dasgupta Lab |
| Last Name: | Dasgupta |
| First Name: | Biplab |
| Address: | Cincinnati, OH |
| Email: | biplab.dasgupta@cchmc.org |
| Phone: | 513-803-1370 |
Subject:
| Subject ID: | SU000790 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | NPC source |
|---|---|---|
| SA042081 | S00026132 | forebrain |
| SA042082 | S00026133 | forebrain |
| SA042083 | S00026134 | hindbrain |
| SA042084 | S00026135 | hindbrain |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000784 |
| Collection Summary: | - |
| Sample Type: | Stem cells |
Treatment:
| Treatment ID: | TR000804 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000797 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_-_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001213 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520B QTOF |
| Ion Mode | NEGATIVE |
| Units | pmol/mg protein |
Chromatography:
| Chromatography ID: | CH000850 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001108 |
| Analysis ID: | AN001213 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
