Summary of Study ST000768
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000554. The data can be accessed directly via it's Project DOI: 10.21228/M8295H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000768 |
| Study Title | Metabolites of human neurons and stem cells (siNeuron metabolomics) |
| Study Type | MS analysis |
| Study Summary | There are a total of four samples each for this analysis. Each sample consists of the cells grown on three 10 cm culture plates. Each plate should have 2x106 cells for a total of 6x106 cells per sample when all three plates are combined. The first sample is undifferentiated human embryonic stem cells, the second sample is human glutamatergic neurons derived from those human embryonic stem cells, the third sample is undifferentiated human induced pluripotent stem cells and the fourth sample is human glutamatergic neurons derived from those human induced pluripotent stem cells. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 4 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000554 |
| Project DOI: | doi: 10.21228/M8295H |
| Project Title: | Metabolites of human neurons and stem cells (siNeuron metabolomics) |
| Project Type: | MS analysis |
| Project Summary: | metabolites of human neurons and stem cells |
| Institute: | University of Michigan |
| Department: | Mol Behav Neurosci Inst |
| Laboratory: | Uhler Lab |
| Last Name: | Uhler |
| First Name: | Michael |
| Address: | Ann Arbor, MI |
| Email: | muhler@umich.edu |
| Phone: | 734-657-3192 |
Subject:
| Subject ID: | SU000791 |
| Subject Type: | HUMAN |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Media | Type |
|---|---|---|---|
| SA042085 | S00026211 | 3N media | neurons |
| SA042086 | S00026213 | 3N media | neurons |
| SA042087 | S00026212 | E8 media | stem cells |
| SA042088 | S00026210 | E8 media | stem cells |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000785 |
| Collection Summary: | - |
| Sample Type: | Stem cells |
Treatment:
| Treatment ID: | TR000805 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000798 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001214 | AN001215 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000851 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001109 |
| Analysis ID: | AN001214 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001110 |
| Analysis ID: | AN001215 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
