Summary of Study ST000769
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000555. The data can be accessed directly via it's Project DOI: 10.21228/M8XM3M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000769 |
| Study Title | Metabolomics of Lin-Sca1+Kit+ (LSK) cells in NM mice |
| Study Type | MS analysis |
| Study Summary | LSKs from transgenic mice are harvested and sorted via flow cytometry. They are collected after two sorts as DAPI-, Lineage-, Sca1+, c-kit+ cells. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000555 |
| Project DOI: | doi: 10.21228/M8XM3M |
| Project Title: | Oncogenic Ras-induced metabolic perturbations |
| Project Type: | MS analysis |
| Project Summary: | Oncogenic Ras-induced metabolic perturbations |
| Institute: | University of Michigan |
| Department: | Internal Med and Cellular and Molecular Biology |
| Laboratory: | Li, Qing Lab |
| Last Name: | Li |
| First Name: | Qing |
| Address: | Ann Arbor, MI |
| Email: | lqing@umich.edu |
| Phone: | 734-763-8624 |
Subject:
| Subject ID: | SU000792 |
| Subject Type: | Mouse |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Type |
|---|---|---|
| SA042090 | S00026009 | mutant ras |
| SA042089 | S00026008 | WT ras |
| Showing results 1 to 2 of 2 |
Collection:
| Collection ID: | CO000786 |
| Collection Summary: | - |
| Sample Type: | Bone marrow |
Treatment:
| Treatment ID: | TR000806 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000799 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_-_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001216 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890N |
| Column | Agilent HP5-MS (15m × 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975 |
| Ion Mode | POSITIVE |
| Units | uM/ng protein |
Chromatography:
| Chromatography ID: | CH000852 |
| Instrument Name: | Agilent 7890N |
| Column Name: | Agilent HP5-MS (15m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001111 |
| Analysis ID: | AN001216 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
