Summary of Study ST000771

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000557. The data can be accessed directly via it's Project DOI: 10.21228/M8P39J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000771
Study TitleLooking for changes in lipid levels with knock out of ACADM (shACADM)
Study TypeMS analysis
Study SummaryGSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8P39J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000557
Project DOI:doi: 10.21228/M8P39J
Project Title:Changes in lipid levels with knock out of ACADM (shACADM) Lipidomics
Project Type:MS analysis
Project Summary:Looking for changes in lipid levels with knock out of ACADM
Institute:University of Michigan
Department:Molecular and Integrative Physiology (MCTP)
Laboratory:Lyssiotis Lab
Last Name:Lyssiotis
First Name:Costas
Address:Ann Arbor, MI
Email:clyssiot@umich.edu
Phone:734-615-9133

Subject:

Subject ID:SU000794
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Transduced with
SA042191S00021734shACADM4
SA042192S00021733shACADM4
SA042193S00021731shACADM4
SA042194S00021732shACADM4
SA042195S00021738shACADM6
SA042196S00021737shACADM6
SA042197S00021736shACADM6
SA042198S00021735shACADM6
SA042199S00021742shACADMscr
SA042200S00021740shACADMscr
SA042201S00021739shACADMscr
SA042202S00021741shACADMscr
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000788
Collection Summary:-
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000808
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000801
Sampleprep Summary:-
Sampleprep Protocol Filename:A008_-_Acyl_Carnitines.pdf

Combined analysis:

Analysis ID AN001218
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Xbridge BEH C18 (50 x 1mm,3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode POSITIVE
Units pmol/ug protein

Chromatography:

Chromatography ID:CH000854
Instrument Name:Agilent
Column Name:Waters Xbridge BEH C18 (50 x 1mm,3.5um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001113
Analysis ID:AN001218
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
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