Summary of study ST000771

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000557. The data can be accessed directly via it's Project DOI: 10.21228/M8P39J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000771
Study TitleLooking for changes in lipid levels with knock out of ACADM (shACADM)
Study TypeMS analysis
Study SummaryGSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups3
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s).D
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8P39J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000557
Project DOI:doi: 10.21228/M8P39J
Project Title:Changes in lipid levels with knock out of ACADM (shACADM) Lipidomics
Project Type:MS analysis
Project Summary:Looking for changes in lipid levels with knock out of ACADM
Institute:University of Michigan
Department:Molecular and Integrative Physiology (MCTP)
Laboratory:Lyssiotis Lab
Last Name:Lyssiotis
First Name:Costas
Address:Ann Arbor, MI
Email:clyssiot@umich.edu
Phone:734-615-9133
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