Summary of Study ST000775
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000561. The data can be accessed directly via it's Project DOI: 10.21228/M8539W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000775 |
| Study Title | Michigan Biomarkers for Refractory Depression (Bluebird) metabolomics pilot study |
| Study Type | MS analysis |
| Study Summary | The goal of the project is discovery of clinical useful biomarkers for treatment-resistant depression. All participants were undergoing electroconvulsive therapy (ECT) for treatment-resistant depression (major depressive disorder or bipolar disorder) in 2012-2014. All subjects were Caucasian except BB060, who was Asian. Samples were collected from each subject at baseline just before the first ECT treatment (Time Point T0) and approximately 3 weeks later just before the tenth ECT treatment (Time Point T2). Whole blood was drawn in the morning in a fasted state (at least 7 hours) through an intravenous catheter or butterfly needle into 6-mL EDTA tubes (Lavender Top, Becton Dickinson Vacutainer K2EDTA additive blood collection tube, part #367863) and transported to the processing lab at the Michigan Clinical Research Unit within 30 minutes. Plasma was isolated by centrifugation for 10 minutes at 2000G at 4C. Plasma from multiple tubes was pooled and aliquotted in 200 uL volumes into 2-mL screw-cap cryovials and frozen at -80C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 29 |
| Total Subjects | 17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000561 |
| Project DOI: | doi: 10.21228/M8539W |
| Project Title: | Michigan Biomarkers for Refractory Depression (Bluebird) |
| Project Type: | MS analysis |
| Project Summary: | Discovery of clinical useful biomarkers for treatment-resistant depression |
| Institute: | University of Michigan |
| Department: | Psychiatry |
| Laboratory: | Mickey Lab |
| Last Name: | Mickey |
| First Name: | Brian |
| Address: | Ann Arbor, MI |
| Email: | bmickey@umich.edu |
| Phone: | 734-647-8239 |
Subject:
| Subject ID: | SU000798 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Pair | Timepoint | Treatment response |
|---|---|---|---|---|
| SA042318 | S00017715 | Pair 1 | T0 | No |
| SA042319 | S00017717 | Pair 1 | T0 | Yes |
| SA042320 | S00017716 | Pair 1 | T2 | No |
| SA042321 | S00017718 | Pair 1 | T2 | Yes |
| SA042322 | S00017719 | Pair 2 | T0 | No |
| SA042323 | S00017721 | Pair 2 | T0 | Yes |
| SA042324 | S00017720 | Pair 2 | T2 | No |
| SA042325 | S00017722 | Pair 2 | T2 | Yes |
| SA042326 | S00017723 | Pair 3 | T0 | No |
| SA042327 | S00017725 | Pair 3 | T0 | Yes |
| SA042328 | S00017724 | Pair 3 | T2 | No |
| SA042329 | S00017726 | Pair 3 | T2 | Yes |
| SA042330 | S00017727 | Pair 4 | T0 | No |
| SA042331 | S00017729 | Pair 4 | T0 | Yes |
| SA042332 | S00017728 | Pair 4 | T2 | No |
| SA042333 | S00017730 | Pair 4 | T2 | Yes |
| SA042334 | S00017731 | Pair 5 | T0 | No |
| SA042335 | S00017733 | Pair 5 | T0 | Yes |
| SA042336 | S00017732 | Pair 5 | T2 | No |
| SA042337 | S00017734 | Pair 5 | T2 | Yes |
| SA042338 | S00017735 | Pair 6 | T0 | No |
| SA042339 | S00017737 | Pair 6 | T0 | Yes |
| SA042340 | S00017736 | Pair 6 | T2 | No |
| SA042341 | S00017738 | Pair 6 | T2 | Yes |
| SA042342 | S00017739 | Pair 7 | T0 | No |
| SA042343 | S00017741 | Pair 7 | T0 | Yes |
| SA042344 | S00017740 | Pair 7 | T2 | No |
| SA042345 | S00017742 | Pair 7 | T2 | Yes |
| SA042346 | S00017743 | Pair 8 | T0 | No |
| SA042347 | S00017745 | Pair 8 | T0 | Yes |
| SA042348 | S00017744 | Pair 8 | T2 | No |
| SA042349 | S00017746 | Pair 8 | T2 | Yes |
| SA042350 | S00020147 | Pooled | Pooled | Pooled |
| Showing results 1 to 33 of 33 |
Collection:
| Collection ID: | CO000792 |
| Collection Summary: | - |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000812 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000805 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001222 | AN001223 | AN001224 | AN001225 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | Triple TOF | Triple TOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | peak area | peak area | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000858 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000859 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001117 |
| Analysis ID: | AN001222 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001118 |
| Analysis ID: | AN001223 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS001119 |
| Analysis ID: | AN001224 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS001120 |
| Analysis ID: | AN001225 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
