Summary of Study ST000780

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000566. The data can be accessed directly via it's Project DOI: 10.21228/M8H979 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000780
Study TitleHigh fat diet and sciatic nerves functions
Study TypeMS analysis
Study SummarySix strain/genotype combinations (BKS wt, BKS db/+, ?) were fed control (5LOD) or high fat (54 % lard) chow from 5 to 36 weeks of age. Longitudinal changes in small and large nerve fiber function, glucose tolerance, fasting blood glucose, body weight etc. were assessed in all groups
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups8
Total Subjects48
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8H979
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000566
Project DOI:doi: 10.21228/M8H979
Project Title:High fat diet and neuropathy studies
Project Type:MS analysis
Project Summary:High-fat diet induced neuropathy -strain comparison
Institute:University of Michigan
Department:Neurology
Laboratory:Feldman Lab
Last Name:Feldman
First Name:Eva
Address:Ann Arbor, MI
Email:efeldman@umich.edu
Phone:734-763-7274

Subject:

Subject ID:SU000803
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id diet genotype strain
SA042398S00016532Control db+ BKS
SA042399S00016530Control db+ BKS
SA042400S00016531Control db+ BKS
SA042401S00016533Control db+ BKS
SA042402S00016548Control db+ BL6
SA042403S00016549Control db+ BL6
SA042404S00016547Control db+ BL6
SA042405S00016546Control db+ BL6
SA042406S00016562Control ob+ BTBR
SA042407S00016563Control ob+ BTBR
SA042408S00016565Control ob+ BTBR
SA042409S00016564Control ob+ BTBR
SA042410S00016522Control wt BKS
SA042411S00016525Control wt BKS
SA042412S00016523Control wt BKS
SA042413S00016524Control wt BKS
SA042414S00016540Control wt BL6
SA042415S00016541Control wt BL6
SA042416S00016538Control wt BL6
SA042417S00016539Control wt BL6
SA042418S00016556Control wt BTBR
SA042419S00016555Control wt BTBR
SA042420S00016554Control wt BTBR
SA042421S00016557Control wt BTBR
SA042422S00016534High Fat db+ BKS
SA042423S00016536High Fat db+ BKS
SA042424S00016535High Fat db+ BKS
SA042425S00016537High Fat db+ BKS
SA042426S00016553High Fat db+ BL6
SA042427S00016550High Fat db+ BL6
SA042428S00016552High Fat db+ BL6
SA042429S00016551High Fat db+ BL6
SA042430S00016568High Fat ob+ BTBR
SA042431S00016566High Fat ob+ BTBR
SA042432S00016567High Fat ob+ BTBR
SA042433S00016569High Fat ob+ BTBR
SA042434S00016527High Fat wt BKS
SA042435S00016528High Fat wt BKS
SA042436S00016526High Fat wt BKS
SA042437S00016529High Fat wt BKS
SA042438S00016542High Fat wt BL6
SA042439S00016544High Fat wt BL6
SA042440S00016545High Fat wt BL6
SA042441S00016543High Fat wt BL6
SA042442S00016561High Fat wt BTBR
SA042443S00016558High Fat wt BTBR
SA042444S00016559High Fat wt BTBR
SA042445S00016560High Fat wt BTBR
Showing results 1 to 48 of 48

Collection:

Collection ID:CO000797
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000817
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000810
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN001234 AN001235
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode NEGATIVE POSITIVE
Units peak area normalized peak area normalized

Chromatography:

Chromatography ID:CH000864
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

MS:

MS ID:MS001129
Analysis ID:AN001234
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
  
MS ID:MS001130
Analysis ID:AN001235
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
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