Summary of Study ST000820

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000584. The data can be accessed directly via it's Project DOI: 10.21228/M85X0R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000820
Study Title2 hydroxyglutarate prodution in neurospheres from IDH1 mouse glioma model #2
Study TypeMS analysis
Study SummaryEvaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from the mice brain tumors haboring specific genetic lesions: NRAS overexpression, p53 knockdown plus or minus IDH1 mutated and ATRX knockdown.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-07-14
Num Groups2
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85X0R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000584
Project DOI:doi: 10.21228/M85X0R
Project Title:2HG prodution in neurospheres from IDH1 mouse glioma model
Project Type:MS analysis
Project Summary:Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from the mice brain tumors haboring specific genetic lesions: NRAS overexpression, p53 knockdown plus or minus IDH1 mutated and ATRX knockdown.
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000846
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id IDH1_MUTATION
SA045810S00026220IDH1-R132H
SA045811S00026221IDH1-R132H
SA045812S00026219IDH1-R132H
SA045813S00026218IDH1-R132H
SA045814S00026216IDH1-R132H
SA045815S00026217IDH1-R132H
SA045816S00026215NO
SA045817S00026214NO
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000840
Collection Summary:-
Sample Type:Glioma cells

Treatment:

Treatment ID:TR000860
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000853
Sampleprep Summary:-

Combined analysis:

Analysis ID AN001303
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode NEGATIVE
Units uM

Chromatography:

Chromatography ID:CH000912
Instrument Name:Agilent
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Solvent A:100% water; 2 mM ammonium formate
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS001196
Analysis ID:AN001303
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A037-2HG_cells_updated-20160721.pdf
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