Summary of Study ST000820
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000584. The data can be accessed directly via it's Project DOI: 10.21228/M85X0R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000820 |
| Study Title | 2 hydroxyglutarate prodution in neurospheres from IDH1 mouse glioma model #2 |
| Study Type | MS analysis |
| Study Summary | Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from the mice brain tumors haboring specific genetic lesions: NRAS overexpression, p53 knockdown plus or minus IDH1 mutated and ATRX knockdown. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-07-14 |
| Num Groups | 2 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000584 |
| Project DOI: | doi: 10.21228/M85X0R |
| Project Title: | 2HG prodution in neurospheres from IDH1 mouse glioma model |
| Project Type: | MS analysis |
| Project Summary: | Evaluation of 2HG and a-ketoglutarate in tumor neurospheres (NS) genertated from the mice brain tumors haboring specific genetic lesions: NRAS overexpression, p53 knockdown plus or minus IDH1 mutated and ATRX knockdown. |
| Institute: | University of Michigan |
| Department: | Neurosurgery |
| Laboratory: | Castro Lab |
| Last Name: | Castro |
| First Name: | Maria |
| Address: | Ann Arbor, MI |
| Email: | mariacas@umich.edu |
| Phone: | 734-764-0850 |
Subject:
| Subject ID: | SU000846 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | IDH1_MUTATION |
|---|---|---|
| SA045810 | S00026220 | IDH1-R132H |
| SA045811 | S00026221 | IDH1-R132H |
| SA045812 | S00026219 | IDH1-R132H |
| SA045813 | S00026218 | IDH1-R132H |
| SA045814 | S00026216 | IDH1-R132H |
| SA045815 | S00026217 | IDH1-R132H |
| SA045816 | S00026215 | NO |
| SA045817 | S00026214 | NO |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000840 |
| Collection Summary: | - |
| Sample Type: | Glioma cells |
Treatment:
| Treatment ID: | TR000860 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000853 |
| Sampleprep Summary: | - |
Combined analysis:
| Analysis ID | AN001303 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490A QQQ |
| Ion Mode | NEGATIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000912 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Solvent A: | 100% water; 2 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001196 |
| Analysis ID: | AN001303 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A037-2HG_cells_updated-20160721.pdf |
