Summary of Study ST000824

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000586. The data can be accessed directly via it's Project DOI: 10.21228/M8XD4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000824
Study Title2HG concentration in human IDH1m cells
Study TypeMS analysis
Study SummarySJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of this project is analyze the production of 2HG in the stable transfected cells (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-07-14
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8XD4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000586
Project DOI:doi: 10.21228/M8XD4X
Project Title:Production of 2HG of human glioma cells
Project Type:MS analysis
Project Summary:Evaluate the concentration of 2HG in human glioma cells harboring IDH1-R132H mutation
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000850
Subject Type:HUMAN
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: HUMAN; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line IDH1-R132H EXPRESSION IDH1-R132H INHIBITOR
SA045855S00028190MGG8 NO NO
SA045856S00028188MGG8 NO NO
SA045857S00028189MGG8 NO NO
SA045858S00028202MGG8 NO YES
SA045859S00028201MGG8 NO YES
SA045860S00028200MGG8 NO YES
SA045861S00028186MGG8 YES NO
SA045862S00028185MGG8 YES NO
SA045863S00028187MGG8 YES NO
SA045864S00028199MGG8 YES YES
SA045865S00028198MGG8 YES YES
SA045866S00028197MGG8 YES YES
SA045867S00028184SJGBM2 NO NO
SA045868S00028183SJGBM2 NO NO
SA045869S00028182SJGBM2 NO NO
SA045870S00028196SJGBM2 NO YES
SA045871S00028195SJGBM2 NO YES
SA045872S00028194SJGBM2 NO YES
SA045873S00028179SJGBM2 YES NO
SA045874S00028181SJGBM2 YES NO
SA045875S00028180SJGBM2 YES NO
SA045876S00028191SJGBM2 YES YES
SA045877S00028192SJGBM2 YES YES
SA045878S00028193SJGBM2 YES YES
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000844
Collection Summary:-
Sample Type:Glioma cells

Treatment:

Treatment ID:TR000864
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000857
Sampleprep Summary:-
Sampleprep Protocol Filename:A037-2HG_cells_updated-20160721.pdf

Combined analysis:

Analysis ID AN001310
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode NEGATIVE
Units uM/ng protein

Chromatography:

Chromatography ID:CH000917
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Solvent A:100% water; 2mM ammonium formate, pH 3.3-4.01,adjust with formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:HILIC

MS:

MS ID:MS001203
Analysis ID:AN001310
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A037-2HG_cells_updated-20160721.pdf
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