Summary of study ST000824

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000586. The data can be accessed directly via it's Project DOI: 10.21228/M8XD4X This work is supported by NIH grant, U2C- DK119886.


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Study IDST000824
Study Title2HG concentration in human IDH1m cells
Study TypeMS analysis
Study SummarySJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of this project is analyze the production of 2HG in the stable transfected cells (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-07-14
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Maureen Kachman Maureen Kachman application/zip

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Project ID:PR000586
Project DOI:doi: 10.21228/M8XD4X
Project Title:Production of 2HG of human glioma cells
Project Type:MS analysis
Project Summary:Evaluate the concentration of 2HG in human glioma cells harboring IDH1-R132H mutation
Institute:University of Michigan
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI