Summary of study ST000824

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000586. The data can be accessed directly via it's Project DOI: 10.21228/M8XD4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000824
Study Title2HG concentration in human IDH1m cells
Study TypeMS analysis
Study SummarySJGBM2 and MGG8 glioma cells were stable transfected with IDH1-R132H mutated. The expression of IDH1-R132H was confirmed by Western Blot assay. The aim of this project is analyze the production of 2HG in the stable transfected cells (SJGBM-IDH1m and MGG8-IDH1m) compared with the control group WT.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-07-14
Num Groups4
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8XD4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000586
Project DOI:doi: 10.21228/M8XD4X
Project Title:Production of 2HG of human glioma cells
Project Type:MS analysis
Project Summary:Evaluate the concentration of 2HG in human glioma cells harboring IDH1-R132H mutation
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850
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