Summary of Study ST000827
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000589. The data can be accessed directly via it's Project DOI: 10.21228/M8J67K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000827 |
| Study Title | Metabolic analysis of Normal Mouse Lung Fiboblasts with/without TGFbeta treatment |
| Study Type | MS analysis |
| Study Summary | Glycolysis/TCA/Nucleotide analysis (especially interested in alpha-ketoglutarate) and NAD+ and related metabolite analysis for parp1 ko and parp1 wild type lung tissue after saline or bleomycin treatment |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 2 |
| Total Subjects | 30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-10-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000589 |
| Project DOI: | doi: 10.21228/M8J67K |
| Project Title: | Metabolic regulation by PARP-1 in myofibroblast differentiation and fibrosis |
| Project Type: | MS analysis |
| Project Summary: | Analysis of wild type lung tissue after saline or bleomycin treatment |
| Institute: | University of Michigan |
| Department: | Deaprtment of Pathology |
| Laboratory: | Sem H. Phan |
| Last Name: | Hu |
| First Name: | Biao |
| Address: | Ann Arbor, MI |
| Email: | biaohu@med.umich.edu |
| Phone: | 734-7635731 |
Subject:
| Subject ID: | SU000853 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Bleomycin | Parp1 KO | Parp1 WT (129S Background) | Saline |
|---|---|---|---|---|---|
| SA046389 | S00014562 | no | no | yes | yes |
| SA046390 | S00014561 | no | no | yes | yes |
| SA046391 | S00014571 | no | no | yes | yes |
| SA046392 | S00014570 | no | no | yes | yes |
| SA046393 | S00014581 | no | no | yes | yes |
| SA046394 | S00014560 | no | no | yes | yes |
| SA046395 | S00014582 | no | no | yes | yes |
| SA046396 | S00014583 | no | no | yes | yes |
| SA046397 | S00014554 | no | yes | no | yes |
| SA046398 | S00014577 | no | yes | no | yes |
| SA046399 | S00014575 | no | yes | no | yes |
| SA046400 | S00014576 | no | yes | no | yes |
| SA046401 | S00014566 | no | yes | no | yes |
| SA046402 | S00014567 | no | yes | no | yes |
| SA046403 | S00014556 | no | yes | no | yes |
| SA046404 | S00014555 | no | yes | no | yes |
| SA046405 | S00014574 | yes | no | yes | no |
| SA046406 | S00014563 | yes | no | yes | no |
| SA046407 | S00014573 | yes | no | yes | no |
| SA046408 | S00014572 | yes | no | yes | no |
| SA046409 | S00014565 | yes | no | yes | no |
| SA046410 | S00014564 | yes | no | yes | no |
| SA046411 | S00014580 | yes | yes | no | no |
| SA046412 | S00014579 | yes | yes | no | no |
| SA046413 | S00014569 | yes | yes | no | no |
| SA046414 | S00014557 | yes | yes | no | no |
| SA046415 | S00014558 | yes | yes | no | no |
| SA046416 | S00014559 | yes | yes | no | no |
| SA046417 | S00014568 | yes | yes | no | no |
| SA046418 | S00014578 | yes | yes | no | no |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO000847 |
| Collection Summary: | - |
| Sample Type: | Fibroblasts |
Treatment:
| Treatment ID: | TR000867 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000860 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A010 - Ceramides.pdf |
Combined analysis:
| Analysis ID | AN001315 | AN001316 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Phenyl | Phenyl |
| Chromatography system | Agilent | Agilent |
| Column | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490A QQQ | Agilent 6490A QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | counts | ng/mg |
Chromatography:
| Chromatography ID: | CH000920 |
| Instrument Name: | Agilent |
| Column Name: | Restek Pinnacle DB Biphenyl (50 x 2.1mm,1.9um,140A) |
| Column Temperature: | 40 |
| Flow Rate: | 200ul/min |
| Solvent A: | 100% water; 5 mM ammonium acetate,pH 9.9 |
| Solvent B: | 60% acetonitrile/40% isopropanol |
| Chromatography Type: | Phenyl |
MS:
| MS ID: | MS001207 |
| Analysis ID: | AN001315 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A010_Ceramides.pdf |
| MS ID: | MS001208 |
| Analysis ID: | AN001316 |
| Instrument Name: | Agilent 6490A QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A010_Ceramides.pdf |
