Summary of Study ST000830

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000591. The data can be accessed directly via it's Project DOI: 10.21228/M88Q3R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000830
Study TitleCD47 WT/KO muscle and serum acyl-carnitine assay
Study TypeMS analysis
Study SummaryThese mice were fed a high-fat diet for 3 weeks, fast for 5 hours, dissected, and quadriceps were snap-frozen in LN2.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups7
Total Subjects9
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-10-11
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M88Q3R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000591
Project DOI:doi: 10.21228/M88Q3R
Project Title:CD47 (integrin associated protein) metabolomics
Project Type:MS analysis
Project Summary:CD47 WT/KO quad/serum (RNAseq mice)
Institute:University of Michigan
Department:Metabolism, Endo & Diabetes
Laboratory:Chun Lab
Last Name:Chun
First Name:Taehwa
Address:Ann Arbor, MI
Email:taehwa@med.umich.edu
Phone:734-763-3056

Subject:

Subject ID:SU000856
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Sample type Type
SA046465S00020332Pooled sample Pooled quadriceps muscle
SA046466S00020333Pooled sample Pooled serum
SA046467S00019650Quadriceps Muscle KO
SA046468S00019649Quadriceps Muscle KO
SA046469S00019651Quadriceps Muscle KO
SA046470S00019648Quadriceps Muscle KO
SA046471S00019645Quadriceps Muscle WT
SA046472S00019644Quadriceps Muscle WT
SA046473S00019646Quadriceps Muscle WT
SA046474S00019647Quadriceps Muscle WT
SA046475S00019658Serum KO
SA046476S00019657Serum KO
SA046477S00019656Serum KO
SA046478S00019659Serum KO
SA046479S00019653Serum WT
SA046480S00019652Serum WT
SA046481S00019654Serum WT
SA046482S00019655Serum WT
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000850
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000870
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000863
Sampleprep Summary:-
Sampleprep Protocol Filename:A008_Acyl_Carnitines.pdf

Combined analysis:

Analysis ID AN001323 AN001324
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent Agilent
Column Waters Xbridge BEH C18 (50 x 1mm,3.5um) Waters Xbridge BEH C18 (50 x 1mm,3.5um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490A QQQ Agilent 6490A QQQ
Ion Mode POSITIVE POSITIVE
Units pmol/mg uM

Chromatography:

Chromatography ID:CH000924
Instrument Name:Agilent
Column Name:Waters Xbridge BEH C18 (50 x 1mm,3.5um)
Column Temperature:40
Flow Rate:0.250 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS001215
Analysis ID:AN001323
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
  
MS ID:MS001216
Analysis ID:AN001324
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Analysis Protocol File:A008_-_Acyl_Carnitines.pdf
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