Summary of Study ST000831

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000592. The data can be accessed directly via it's Project DOI: 10.21228/M84X1T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000831
Study TitleBile acid analysis of the germ free mouse gut
Study TypeMS analysis
Study SummaryWe are interested in understanding the bile acid profiles from the feces of fecal microbiota transplant FMT patients that successfully recover from recurrent C. difficile infection. We have are submitting fecal samples from patients prior to their FMT and post FMT. We are also interested in the bile acid profiles of the donor stool that is used in successful transplants. Bile acids are important for C. difficile spore germination and outgrowth.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups6
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M84X1T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000592
Project DOI:doi: 10.21228/M84X1T
Project Title:The role of the gut microbiota in secondary bile acid metabolism
Project Type:MS analysis
Project Summary:Targeted bile acid metabolomics
Institute:North Carolina State University
Department:Population Health and Pathobiology
Laboratory:Theriot Lab
Last Name:Theriot
First Name:Casey
Address:Raleigh, NC
Email:caseythe@umich.edu
Phone:734-763-4133

Subject:

Subject ID:SU000857
Subject Type:MOUSE
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id GENDER Sample type
SA046483S00020468female Cecum
SA046484S00020472female Cecum
SA046485S00020473female Colon
SA046486S00020469female Colon
SA046487S00020471female Ileum
SA046488S00020467female Ileum
SA046489S00020470female Serum
SA046490S00020466female Serum
SA046491S00020460male Cecum
SA046492S00020464male Cecum
SA046493S00020461male Colon
SA046494S00020465male Colon
SA046495S00020459male Ileum
SA046496S00020463male Ileum
SA046497S00020458male Serum
SA046498S00020462male Serum
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000851
Collection Summary:-
Sample Type:Feces

Treatment:

Treatment ID:TR000871
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000864
Sampleprep Summary:-
Sampleprep Protocol Filename:A014_BileAcids.pdf

Combined analysis:

Analysis ID AN001325
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent
Column Waters Xbridge BEH C18 (150 x 2.1mm,3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490A QQQ
Ion Mode NEGATIVE
Units ug/100 mg

Chromatography:

Chromatography ID:CH000925
Instrument Name:Agilent
Column Name:Waters Xbridge BEH C18 (150 x 2.1mm,3.5um)
Flow Rate:200ul/min
Solvent A:75% acetonitrile/40% methanol; 10mM ammonium acetate
Solvent B:100% water; 10 mM ammonium acetate, pH 8.0
Chromatography Type:Reversed phase

MS:

MS ID:MS001217
Analysis ID:AN001325
Instrument Name:Agilent 6490A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
Analysis Protocol File:A014_BileAcids.pdf
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