Summary of study ST000832

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000593. The data can be accessed directly via it's Project DOI: 10.21228/M81689 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000832
Study TitleRat Retinal Detachment Metabolomics Timecourse
Study TypeMS analysis
Study SummaryDetachments were created in adult male Brown-Norway rats (300?400 g; Charles River Laboratories, Wilmington, MA). Briefly, rodents were anesthetized with a 50:50 mix of ketamine/xylazine, and pupils were dilated with topical phenylephrine (2.5%) and tropicamide (1%). A 20-gauge microvitreoretinal blade was used to create a sclerotomy 2 mm posterior to the limbus, carefully avoiding lens damage. A subretinal injector (Glaser, 32-gauge tip; BD Ophthalmic Systems, Franklin Lakes, NJ) was introduced through the sclerotomy into the vitreous cavity and then through a peripheral retinotomy into the subretinal space. Sodium hyaluronate (10 mg/mL, Healon OVD; Abbott Medical Optics, Uppsala, Sweden) was slowly injected to detach the neurosensory retina from the underlying RPE. In all experiments, approximately one third to one half of the neurosensory retina was detached. Detachments were created in the left eye. The right eye served as the control, with all the steps of the procedure performed except for introduction of the subretinal injector and injection of the sodium hyaluronate. At varying intervals after creation of the detachment, the animals were euthanatized, and the eyes were enucleated.The retina was then dissected away from the retinal pigment epithelium taking just the detached portion in those eyes experimentally detached. All experiments were performed in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and the guidelines established by the University Committee on Use and Care of Animals of the University of Michigan.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups30
Total Subjects21
Raw Data AvailableYes
Analysis Type DetailGC/LC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M81689
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000593
Project DOI:doi: 10.21228/M81689
Project Title:Regulatory roles of glycolysis and its enzymes in photoreceptor survival
Project Type:MS analysis
Project Summary:The goals specific to this proposal are to understand how the metabolic flux through glycolysis and its associated pathways, such as oxidative phosphorylation and pentose phosphate pathway, is regulated during periods of photoreceptor stress.
Institute:University of Michigan
Department:Ophthalmology and Visual Sciences
Laboratory:Besirli Lab
Last Name:Besirli
First Name:Cagri
Address:Ann Arbor, MI
Email:cbesirli@umich.edu
Phone:734-615-6099
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