Summary of Study ST000833
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000594. The data can be accessed directly via it's Project DOI: 10.21228/M8WD50 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000833 |
| Study Title | Influence of murine norovirus on cell metabolism |
| Study Type | MS analysis |
| Study Summary | 6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 2 |
| Total Subjects | 1 |
| Study Comments | BIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2018-08-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000594 |
| Project DOI: | doi: 10.21228/M8WD50 |
| Project Title: | Influence of murine norovirus on cell metabolism |
| Project Type: | MS analysis |
| Project Summary: | C6 effects in RAW |
| Institute: | University of Michigan |
| Department: | Microbiology & Immunology |
| Laboratory: | Passalacqua Lab |
| Last Name: | Passalacqua |
| First Name: | Karla |
| Address: | Ann Arbor, MI |
| Email: | kpassal@umich.edu |
| Phone: | 734-763-5639 |
Subject:
| Subject ID: | SU001180 |
| Subject Type: | MOUSE |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
Subject type: MOUSE; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Medium |
|---|---|---|
| SA077807 | S00023724 | MNV infected |
| SA077808 | S00023723 | MNV infected |
| SA077809 | S00023722 | MNV infected |
| SA077810 | S00023720 | mock lysate |
| SA077811 | S00023721 | mock lysate |
| SA077812 | S00023719 | mock lysate |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO001174 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001195 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP001188 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001841 | AN001842 | AN001843 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | GC | HILIC |
| Chromatography system | Agilent | Agilent GC_7890N | Agilent |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent HP5-MS (15m × 0.25mm, 0.25 um) | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI | EI | ESI |
| MS instrument type | QTOF | Single quadrupole | QTOF |
| MS instrument name | Agilent 6520B QTOF | Agilent MS_5975 | Agilent 6520B QTOF |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | uM | pmol/ug protein | counts |
Chromatography:
| Chromatography ID: | CH001318 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Flow Rate: | 0.075 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001319 |
| Instrument Name: | Agilent GC_7890N |
| Column Name: | Agilent HP5-MS (15m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
| Chromatography ID: | CH001320 |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001702 |
| Analysis ID: | AN001841 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m |
| Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001703 |
| Analysis ID: | AN001842 |
| Instrument Name: | Agilent MS_5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | HEIDIFAMES.M |
| Analysis Protocol File: | A011_Glycolysis-TCA-nucleotides.pdf |
| MS ID: | MS001704 |
| Analysis ID: | AN001843 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m |
| Analysis Protocol File: | A043-TCA-Plus-20170713.pdf |
