Summary of study ST000833

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000594. The data can be accessed directly via it's Project DOI: 10.21228/M8WD50 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000833
Study TitleInfluence of murine norovirus on cell metabolism
Study TypeMS analysis
Study Summary6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups2
Total Subjects1
Study CommentsBIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data.
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailGC/LC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8WD50
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000594
Project DOI:doi: 10.21228/M8WD50
Project Title:Influence of murine norovirus on cell metabolism
Project Type:MS analysis
Project Summary:C6 effects in RAW
Institute:University of Michigan
Department:Microbiology & Immunology
Laboratory:Passalacqua Lab
Last Name:Passalacqua
First Name:Karla
Address:Ann Arbor, MI
Email:kpassal@umich.edu
Phone:734-763-5639
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