Summary of study ST000833

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000594. The data can be accessed directly via it's Project DOI: 10.21228/M8WD50 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000833
Study TitleInfluence of murine norovirus on cell metabolism
Study TypeMS analysis
Study Summary6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups2
Total Subjects1
Study CommentsBIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data.
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailGC/LC-MS
Release Date2018-08-27
Release Version1
Maureen Kachman Maureen Kachman application/zip

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Project ID:PR000594
Project DOI:doi: 10.21228/M8WD50
Project Title:Influence of murine norovirus on cell metabolism
Project Type:MS analysis
Project Summary:C6 effects in RAW
Institute:University of Michigan
Department:Microbiology & Immunology
Laboratory:Passalacqua Lab
Last Name:Passalacqua
First Name:Karla
Address:Ann Arbor, MI