Summary of Study ST000834
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000595. The data can be accessed directly via it's Project DOI: 10.21228/M8RQ4G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000834 |
| Study Title | Purine and TCA measurements in salt sensitive (SS) hypertension |
| Study Type | MS analysis |
| Study Summary | SS rats were surgically implanted with a chronic servo-control cuff, the purpose of which is to maintain normal pressure to the left kidney while the right kidney is exposed to high blood pressure. After 7 days of high salt treatment, which induces high blood pressure in the SS rat, rats were anesthetized with pentobarbital, kidneys were flushed and removed. The renal medulla was separated from the cortex using scissors, and the renal medullas were frozen in liquid nitrogen and stored at -80 C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-08-02 |
| Num Groups | 11 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000595 |
| Project DOI: | doi: 10.21228/M8RQ4G |
| Project Title: | Purine and TCA Measurements in SS Hypertension |
| Project Type: | MS analysis |
| Project Summary: | Based on RNA-seq ontological and Bayesian analyses, both purine levels and TCA-cycle intermediates have been nominated as being regulated by pressure in the SS rat. This project seeks to verify the previous RNA-seq prediction. |
| Institute: | Medical College of Wisconsin |
| Department: | Physiology |
| Laboratory: | Cowley Lab |
| Last Name: | Cowley |
| First Name: | Allen |
| Address: | Milwaukee, WI |
| Email: | cowley@mcw.edu |
| Phone: | 414-955-8277 |
Subject:
| Subject ID: | SU000910 |
| Subject Type: | RAT |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammal |
Factors:
Subject type: RAT; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Side |
|---|---|---|
| SA050829 | S00025614 | Left |
| SA050830 | S00025608 | Left |
| SA050831 | S00025612 | Left |
| SA050832 | S00025610 | Left |
| SA050833 | S00025609 | Right |
| SA050834 | S00025615 | Right |
| SA050835 | S00025611 | Right |
| SA050836 | S00025613 | Right |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000904 |
| Collection Summary: | - |
| Sample Type: | Kidney |
Treatment:
| Treatment ID: | TR000924 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000917 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A011_Glycolysis-TCA-nucleotides.pdf |
Combined analysis:
| Analysis ID | AN001424 | AN001425 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | GC |
| Chromatography system | Agilent | Agilent 7890N |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) | Agilent HP5-MS (15m × 0.25mm, 0.25 um) |
| MS Type | ESI | EI |
| MS instrument type | QTOF | Single quadrupole |
| MS instrument name | Agilent 6520B QTOF | Agilent 5975 |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | counts/mg | nmol/mg |
Chromatography:
| Chromatography ID: | CH000995 |
| Chromatography Summary: | Amino, Aminopropyl HILIC |
| Instrument Name: | Agilent |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH000996 |
| Chromatography Summary: | 5% Phenyl Methyl Siloxane |
| Instrument Name: | Agilent 7890N |
| Column Name: | Agilent HP5-MS (15m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001314 |
| Analysis ID: | AN001424 |
| Instrument Name: | Agilent 6520B QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m.zip |
| Analysis Protocol File: | A043-TCA-Plus-20170713.pdf |
| MS ID: | MS001315 |
| Analysis ID: | AN001425 |
| Instrument Name: | Agilent 5975 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
| Acquisition Parameters File: | HEIDI_FAMES_10MIN.M.zip |
| Analysis Protocol File: | A011_-_Glycolysis-TCA-nucleotides.pdf |
